Pathophysiology Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Department of Medical Technology and Sciences, School of Health Sciences at Narita, International University of Health and Welfare, Narita, Japan.
Acta Cytol. 2022;66(1):55-60. doi: 10.1159/000519335. Epub 2021 Oct 13.
Liquid-based cytology (LBC) is increasingly used for nongynecologic applications. However, the cytological preparation of LBC specimens is influenced by the processing technique and the preservative used. In this study, the influence of the processing techniques and preservatives on cell morphology was examined mathematically and statistically.
Cytological specimens were prepared using the ThinPrep (TP), SurePath (SP), and AutoSmear methods, with 5 different preservative solutions. The cytoplasmic and nuclear areas of Papanicolaou-stained specimens were measured for all samples.
The cytoplasmic and nuclear areas were smaller in cells prepared using the 2 LBC methods, compared to that prepared using the AutoSmear method, irrespective of the preservative used. The cytoplasmic and nuclear areas of cells prepared using the SP method were smaller than those of cells prepared using the TP method, irrespective of the preservative used. There were fewer differences among the cytoplasmic areas of cells prepared with different preservative solutions using the TP method; however, the cytoplasmic areas of cells prepared using the SP method changed with the preservative solution used.
The most significant difference affecting the cytoplasmic and nuclear areas was the processing technique. The TP method increased the cytoplasmic and nuclear areas, while the methanol-based PreservCyt solution enabled the highest enlargement of the cell. LBC is a superior preparation technique for standardization of the specimens. Our results offer a better understanding of methods suitable for specimen preparation for developing precision AI-based diagnosis in cytology.
液基细胞学（LBC）越来越多地用于非妇科应用。然而，LBC 标本的细胞学制备受到处理技术和使用的防腐剂的影响。在这项研究中，从数学和统计学角度研究了处理技术和防腐剂对细胞形态的影响。
使用 ThinPrep（TP）、SurePath（SP）和 AutoSmear 方法以及 5 种不同的防腐剂溶液制备细胞学标本。对所有样本的巴氏染色标本的细胞质和核区进行测量。
与 AutoSmear 方法相比，无论使用哪种防腐剂，使用 2 种 LBC 方法制备的细胞的细胞质和核区都较小。与使用 TP 方法相比，使用 SP 方法制备的细胞的细胞质和核区较小，无论使用哪种防腐剂。使用 TP 方法制备的不同防腐剂溶液的细胞的细胞质区之间的差异较小；然而，使用 SP 方法制备的细胞的细胞质区随所用防腐剂溶液的变化而变化。
影响细胞质和核区的最大差异是处理技术。TP 方法增加了细胞质和核区，而基于甲醇的 PreservCyt 溶液使细胞的放大率最高。LBC 是一种用于标本标准化的优越制备技术。我们的结果为选择适合细胞学标本制备的方法提供了更好的理解，以开发基于人工智能的细胞学精准诊断。
