Cancer Institute/Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, People's Republic of China.
Cancer Cytopathol. 2011 Dec 25;119(6):387-94. doi: 10.1002/cncy.20177. Epub 2011 Jul 19.
Liquid-based cytology (LBC) has been compared with conventional cytology in numerous studies. In the current study of 2 LBC systems, the accuracy, rates of unsatisfactory cytology, and sufficiency of residual LBC specimens for Hybrid Capture 2 (HC2) HPV DNA testing were compared.
Eligible women ages 30 to 49 years were recruited for this cross-sectional population-based study in rural China. Women were assessed by visual inspection with acetic acid (VIA), LBC, and high-risk HPV HC2 DNA assay. Cervical specimens were preserved according to SurePath or ThinPrep protocols. LBC results were manually read. HC2 testing was performed on specimens with sufficient residual volume. Colposcopies and biopsies were performed on women who were VIA positive at the time of initial screening. Women with abnormal LBC or HC2 test results were called back for colposcopies and 4-quadrant cervical biopsies.
Of 2005 eligible women, 972 were tested by SurePath and 1033 by ThinPrep. Compared with SurePath samples, ThinPrep samples had higher rates of unsatisfactory cytology (0.2% for SurePath and 1.5% for ThinPrep) and insufficient residual volume for HC2 (0.0% for SurePath and 18.2% for ThinPrep). SurePath samples yielded higher sensitivities and similar specificities for LBC and HC2 testing of residual specimens, but these differences were not determined to be significant by area-under-the-curve analysis (LBC performance: 0.89 for SurePath and 0.85 for ThinPrep; HC2 performance: 0.91 for SurePath and 0.89 for ThinPrep).
Both methods yielded similar validity in detecting significant cervical lesions. However, SurePath samples yielded higher rates of satisfactory LBC slides and sufficient residual volume for HC2.
液基细胞学（LBC）已在多项研究中与传统细胞学进行了比较。在本研究中，对 2 种 LBC 系统进行了比较，比较了准确性、不满意细胞学的发生率以及剩余 LBC 标本用于 Hybrid Capture 2（HC2）HPV DNA 检测的充足性。
本研究为在中国农村进行的一项基于人群的横断面研究，纳入了年龄在 30 至 49 岁之间的合格女性。对这些女性进行了醋酸视觉检查（VIA）、LBC 和高危型 HPV HC2 DNA 检测。根据 SurePath 或 ThinPrep 方案保存宫颈标本。LBC 结果由人工读取。剩余体积足够的标本进行 HC2 检测。在初次筛查时 VIA 阳性的女性进行阴道镜检查和活检。对 LBC 或 HC2 检测结果异常的女性进行召回，进行阴道镜检查和 4 象限宫颈活检。
在 2005 名合格女性中，972 名接受了 SurePath 检测，1033 名接受了 ThinPrep 检测。与 SurePath 样本相比，ThinPrep 样本的不满意细胞学发生率更高（SurePath 为 0.2%，ThinPrep 为 1.5%），用于 HC2 的剩余标本的体积不足（SurePath 为 0.0%，ThinPrep 为 18.2%）。SurePath 样本对剩余标本的 LBC 和 HC2 检测具有更高的敏感性和相似的特异性，但曲线下面积分析未确定这些差异具有统计学意义（LBC 检测性能：SurePath 为 0.89，ThinPrep 为 0.85；HC2 检测性能：SurePath 为 0.91，ThinPrep 为 0.89）。
两种方法在检测显著宫颈病变方面均具有相似的有效性。然而，SurePath 样本产生了更高比例的满意 LBC 载玻片和足够用于 HC2 的剩余体积。
