Department of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, 287 Changhuai Road, Bengbu, 233004, Anhui, People's Republic of China.
Mol Biotechnol. 2022 Mar;64(3):330-337. doi: 10.1007/s12033-021-00412-w. Epub 2021 Oct 14.
Lower extremity deep venous thrombosis (LEDVT) is a venous reflux disorder caused by abnormal coagulation of blood. LEDVT can obstruct the lumen and LEDVT is the third vascular disease after cerebrovascular diseases and coronary artery diseases. miRNAs are associated with thrombosis, and miR-185 was reported to affect the proliferation and apoptosis of vascular endothelial cells by regulating receptor of advanced glycation end products (RAGE). However, no study has reported the effect of miR-185 on LEDVT. Here, we studied the effects of miR-185 on the PI3K/AKT and MAPK signaling pathways in the LEDVT cells. The results showed that miR-185 promotes cell proliferation through activating the PI3K/AKT and MAPK signaling pathways and then inhibits tissue factor and fibrin expression to reduce thrombosis. In short, our study provides new ideas and a theoretical basis for research on the prevention, diagnosis, and treatment of LEDVT.
下肢深静脉血栓形成（LEDVT）是一种由血液异常凝结引起的静脉回流障碍。LEDVT 可阻塞管腔，是继脑血管病和冠状动脉疾病之后的第三大血管疾病。miRNAs 与血栓形成有关，有研究报道 miR-185 通过调节晚期糖基化终产物受体（RAGE）影响血管内皮细胞的增殖和凋亡。然而，目前尚无研究报道 miR-185 对 LEDVT 的影响。在这里，我们研究了 miR-185 对 LEDVT 细胞中 PI3K/AKT 和 MAPK 信号通路的影响。结果表明，miR-185 通过激活 PI3K/AKT 和 MAPK 信号通路促进细胞增殖，进而抑制组织因子和纤维蛋白表达，减少血栓形成。总之，我们的研究为 LEDVT 的预防、诊断和治疗提供了新的思路和理论依据。
