Department of General surgery, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, China.
Department of Vascular Surgery, Yancheng First People's Hospital of Jiangsu Province, Yancheng, Jiangsu Province, China.
Bioengineered. 2022 May;13(5):13452-13461. doi: 10.1080/21655979.2022.2076496.
Lower extremity deep vein thrombosis (LEDVT) is a disorder of venous return caused by abnormal blood clotting. LEDVT can obstruct the lumen and is the third most common vascular disease after cerebrovascular disease and coronary artery disease. LncRNAs are associated with thrombosis and potentially affect the pathogenesis of DVT. However, no studies have reported the effect of LINC01123 on LEDVT. The aim of this study was to investigate the effect of LINC01123 on LEDVT in rats via the miR-125a-3p/interleukin 1 receptor type 1 (IL1R1) axis. Lentiviral vectors that altering LINC01123, miR-125a-3p and IL1R1 expression were pre-injected into the tail vein of rats, and an LEDVT model was established 1 day later. Detection of LINC01123, miR-125a-3p and IL1R1 expression was performed. Inflammatory factors in femoral venous blood, the length and weight of the thrombus, the histomorphological changes were determined in the rat model. The targeting relation of miR-125a-3p with LINC01123 or IL1R1 was verified. The results presented that LEDVT rats expressed high LINC01123 and IL1R1 and low miR-125a-3p expression levels. After silencing LINC01123 or elevating miR-125a-3p, the rate of thrombosis, length and weight of thrombus, and levels of inflammatory factors were reduced. The targeting relation was presented between miR-125a-3p with LINC01123 or IL1R1. Elevating IL1R1 was available to turn around the action of silence of LINC01123 on LEDVT rats. All in all, suppression of LINC01123 restrains LEDVT via miR-125a-3p to target IL1R1.
下肢深静脉血栓形成（LEDVT）是一种由血液异常凝结引起的静脉回流障碍。LEDVT 可阻塞管腔，是继脑血管病和冠状动脉疾病之后的第三大常见血管疾病。lncRNAs 与血栓形成有关，可能影响 DVT 的发病机制。然而，尚无研究报道 LINC01123 对 LEDVT 的影响。本研究旨在通过 LINC01123/miR-125a-3p/白细胞介素 1 受体 1（IL1R1）轴探讨 LINC01123 对 LEDVT 大鼠的影响。将改变 LINC01123、miR-125a-3p 和 IL1R1 表达的慢病毒载体预先注入大鼠尾静脉，1 天后建立 LEDVT 模型。检测大鼠模型中 LINC01123、miR-125a-3p 和 IL1R1 的表达。检测股静脉血中炎症因子、血栓的长度和重量，以及大鼠的组织形态学变化。验证 miR-125a-3p 与 LINC01123 或 IL1R1 的靶向关系。结果表明，LEDVT 大鼠表达高水平的 LINC01123 和 IL1R1，低水平的 miR-125a-3p。沉默 LINC01123 或升高 miR-125a-3p 后，血栓形成率、血栓长度和重量以及炎症因子水平降低。miR-125a-3p 与 LINC01123 或 IL1R1 之间存在靶向关系。升高 IL1R1 可逆转沉默 LINC01123 对 LEDVT 大鼠的作用。总之，抑制 LINC01123 通过 miR-125a-3p 靶向 IL1R1 抑制 LEDVT。
