白藜芦醇通过激活 Wnt/β-连环蛋白信号通路促进脊髓损伤后的轴突再生。
Resveratrol promotes axonal regeneration after spinal cord injury through activating Wnt/β-catenin signaling pathway.
机构信息
Department of Orthopaedics, The 900th Hospital, Joint Logistics Support Force, Fuzhou 350025, Fujian Province, P.R. China.
Department of Orthopaedics, Fuzong Clinical Medical College of Fujian Medical University, Fuzhou 350025, Fujian Province, P.R. China.
出版信息
Aging (Albany NY). 2021 Oct 14;13(20):23603-23619. doi: 10.18632/aging.203628.
BACKGROUND
Spinal cord injury (SCI) is characterized by autonomic dysreflexia, chronic pain, sensory and motor deficits. Resveratrol has shown potential neuroprotective function in several neurodegenerative diseases' models. However, if resveratrol could improve the function recovery after SCI and the further mechanism have not been investigated.
METHODS
SCI rat model was established through laminectomy at lamina T9-10 aseptically. Basso, beattie and bresnahan (BBB) and inclined plane score, sensory recovery, spinal cord content, and inflammatory factors were measured. The levels of GAP43, NF421, GFAP, Bax, Bcl-2 and caspase-3 were measured using immunohistochemical staining. Tunel staining was applied to detect apoptosis level.
RESULTS
Resveratrol significantly improved the function recovery, promoted axonal regeneration, suppressed apoptosis after SCI. The activation of Wnt/β-catenin signaling pathway was achieved by resveratrol. XAV939 significantly reversed the influence of resveratrol on function recovery, axonal regeneration, apoptosis after SCI.
CONCLUSIONS
Resveratrol could promote the function recovery and axonal regeneration, improve histological damage, inhibit apoptosis level after SCI through regulating Wnt/β-catenin signaling pathway. This research expanded the regulatory mechanism of resveratrol in SCI injury.
背景
脊髓损伤（SCI）的特征是自主反射异常、慢性疼痛、感觉和运动功能缺陷。白藜芦醇在几种神经退行性疾病模型中显示出潜在的神经保护功能。然而，白藜芦醇是否能改善 SCI 后的功能恢复，以及其进一步的机制尚未得到研究。
方法
通过无菌椎板切除术在 T9-10 椎板建立 SCI 大鼠模型。使用 Basso、Beattie 和 Bresnahan（BBB）评分和斜面评分、感觉恢复、脊髓内容物和炎症因子来测量。使用免疫组织化学染色测量 GAP43、NF421、GFAP、Bax、Bcl-2 和 caspase-3 的水平。Tunel 染色用于检测细胞凋亡水平。
结果
白藜芦醇显著改善了 SCI 后的功能恢复，促进了轴突再生，抑制了细胞凋亡。白藜芦醇激活了 Wnt/β-catenin 信号通路。XAV939 显著逆转了白藜芦醇对 SCI 后功能恢复、轴突再生和细胞凋亡的影响。
结论
白藜芦醇通过调节 Wnt/β-catenin 信号通路，促进 SCI 后的功能恢复和轴突再生，改善组织学损伤，抑制细胞凋亡水平。本研究扩展了白藜芦醇在 SCI 损伤中的调控机制。
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