Postdoctoral of Shandong University of Traditional Chinese Medicine, Jinan, China; Department of Orthopedics, Jining No.1 People's Hospital, Jining, China.
Department of Orthopedics, Jining No.2 People's Hospital, Jining, China.
Brain Res. 2024 Jan 1;1822:148637. doi: 10.1016/j.brainres.2023.148637. Epub 2023 Oct 17.
The specific molecular mechanism of neuroprotective effects of wnt-3a on spinal cord injury (SCI) has not been elucidated. In our study, we evaluated the recovery of motor function after SCI by BBB, observed neuronal apoptosis by western blot and TUNEL, observed the changes of neuronal inflammation by western blot and immunofluorescence staining, and observed the changes of motoneurons and spinal cord area in the anterior horn of the spinal cord via Nissl and HE staining. We found that wnt-3a could significantly promote the recovery of motor function, reduce the loss of motor neurons in the anterior horn of the spinal cord, promote the recovery of injured spinal cord tissue, inhibit neuronal apoptosis and inflammatory response, and ultimately promote neuronal function after SCI. However, when XAV939 inhibits the wnt/β-catenin signaling pathway, the neuroprotective effects of wnt-3a are also significantly inhibited. The above results together indicated that wnt-3a exerts its neuroprotective effect on after SCI via activating the wnt/β-catenin signaling pathway.
Wnt-3a 对脊髓损伤（SCI）的神经保护作用的具体分子机制尚未阐明。在我们的研究中，我们通过 BBB 评估 SCI 后的运动功能恢复情况，通过 Western blot 和 TUNEL 观察神经元凋亡情况，通过 Western blot 和免疫荧光染色观察神经元炎症变化，通过尼氏染色和 HE 染色观察脊髓前角运动神经元和脊髓面积的变化。我们发现，wnt-3a 能显著促进运动功能的恢复，减少脊髓前角运动神经元的丢失，促进损伤脊髓组织的恢复，抑制神经元凋亡和炎症反应，最终促进 SCI 后神经元功能的恢复。然而，当 XAV939 抑制 wnt/β-catenin 信号通路时，wnt-3a 的神经保护作用也显著受到抑制。上述结果共同表明，wnt-3a 通过激活 wnt/β-catenin 信号通路对 SCI 后发挥神经保护作用。
