Department of Anesthesiology, Second Affiliated Hospital, Army Medical University, Chongqing 400037, China.
Department of Anesthesiology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China.
Exp Biol Med (Maywood). 2022 Jan;247(2):165-173. doi: 10.1177/15353702211046546. Epub 2021 Oct 14.
Ubiquinol-cytochrome c reductase core protein 1 (UQCRC1) is an indispensable component of mitochondrial complex III. It plays a key role in cardioprotection and maintaining mitochondrion function. However, the exact role of UQCRC1 in maintaining cardiac function has not been reported by models. Also, the exact biological functions of UQCRC1 are far from fully understood. UQCRC1 mice had decreased both mRNA and protein expression of UQCRC1 in the left ventricular myocardia, and these mice had reduced tolerance to acute exhaustive exercise including decreased time and distance with higher apoptosis rate, higher expression level of cleaved CASPASE 3, and higher ratio of cleaved PARP1 to full-length PARP1. Moreover, UQCRC1 knockdown led to increased LV interventricular septal thicknesses both at systole and diastole, as well as decreased LV volume both at end-systole and end-diastole. Finally, UQCRC1 gene disruption resulted in mitochondrial vacuolation, fibril disarrangement, and more severe morphological and structural changes in mitochondria after acute exhaustive exercise. In conclusion, UQCRC1 contributes to cardiac tolerance to acute exhaustive exercise in mice, and it may be an essential component of complex III, playing a crucial role in maintaining cardiac functions.
泛醌细胞色素 c 还原酶核心蛋白 1（UQCRC1）是线粒体复合物 III 不可或缺的组成部分。它在心脏保护和维持线粒体功能方面起着关键作用。然而，UQCRC1 在维持心脏功能中的确切作用尚未通过模型报道。此外，UQCRC1 的确切生物学功能还远未完全理解。UQCRC1 敲除小鼠左心室心肌中的 UQCRC1 mRNA 和蛋白表达均降低，这些小鼠对急性耗竭运动的耐受性降低，包括运动时间和距离缩短，凋亡率升高，caspase 3 剪切水平升高，PARP1 全长与剪切体的比值升高。此外，UQCRC1 敲低导致左室室间隔在收缩期和舒张期的厚度增加，左室在收缩末期和舒张末期的容积减少。最后，UQCRC1 基因缺失导致急性耗竭运动后线粒体空泡化、纤维排列紊乱以及线粒体更严重的形态和结构改变。总之，UQCRC1 有助于小鼠对急性耗竭运动的心脏耐受，它可能是复合物 III 的一个必需组成部分，在维持心脏功能方面起着至关重要的作用。
