Residual Agrochemical Assessment Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Republic of Korea.
PLoS One. 2021 Oct 14;16(10):e0258266. doi: 10.1371/journal.pone.0258266. eCollection 2021.
Traditionally in Korea, Protaetia brevitarsis seulensis (white-spotted flower chafer) has been used as a medicine, and recently has attracted increased attention due to its antithrombotic efficacy. Some of spent mushroom compost or fermented oak sawdust, a feedstock for P. brevitarsis, were contaminated with three fungicides, carbendazim, dimethomorph, and fenoxanil, which could be transferred to the insect. This study was aimed to optimize a simple extraction method combined with liquid chromatography tandem mass spectrometry and apply it to the real samples. After the pulverized samples (5 g) were extracted with acetonitrile (10 mL) and formic acid (100 μL), fat and lipids in the samples were slowly precipitated at -20°C for 24 hours. After eight different clean-up methods were investigated, the mixture of 150 mg MgSO4/25 mg PSA/25 mg C18 was selected due to optimal recovery of the target compounds. Recovery (77.9%‒80.8% for carbendazim, 111.2%‒116.7% for dimethomorph, and 111.9%‒112.5% for fenoxanil) was achieved with reasonable relative standard deviation (<5.5%) The analytical method developed in this study was used to analyze three compounds in the 24 insect samples donated by the insect farm owners but no target compounds were detected. These results can provide important data for establishing the pesticide safety standards for P. brevitarsis before the medical applications.
在韩国，传统上一直将白星花金龟（Protaetia brevitarsis seulensis）用作药材，由于其抗血栓形成的功效，最近受到了越来越多的关注。一些蘑菇废料或发酵橡木锯末（白星花金龟的饲料）被三种杀菌剂（多菌灵、代森锰锌和苯氧菌胺）污染，这些杀菌剂可能会转移到昆虫体内。本研究旨在优化一种简单的提取方法，结合液相色谱串联质谱法，并将其应用于实际样品。将粉碎后的样品（5 g）用乙腈（10 mL）和甲酸（100 μL）提取后，样品中的脂肪和脂质在-20°C下缓慢沉淀 24 小时。在考察了 8 种不同的净化方法后，由于目标化合物的回收率最佳，选择了 150 mg MgSO4/25 mg PSA/25 mg C18 的混合物。回收率（多菌灵为 77.9%‒80.8%，代森锰锌为 111.2%‒116.7%，苯氧菌胺为 111.9%‒112.5%）相对标准偏差（<5.5%）合理。本研究建立的分析方法用于分析昆虫养殖场所有者捐赠的 24 个昆虫样本中的三种化合物，但未检测到目标化合物。这些结果可为建立白星花金龟的农药安全标准提供重要数据，以便在医疗应用之前进行评估。
