Biotechnology, Department of Chemistry, Lund University, PO Box 124, Lund, SE-22100, Sweden.
Biotechnology, Department of Chemistry, Lund University, PO Box 124, Lund, SE-22100, Sweden.
Bioresour Technol. 2022 Jan;343:126114. doi: 10.1016/j.biortech.2021.126114. Epub 2021 Oct 11.
The success of establishing bioeconomies replacing current economies based on fossil resources largely depends on our ability to degrade recalcitrant lignocellulosic biomass. This study explores the potential of employing various enzymes acting synergistically on previously pretreated agricultural side streams (corn bran, oat hull, soluble and insoluble oat bran). Degrees of synergy (oligosaccharide yield obtained with the enzyme combination divided by the sum of yields obtained with individual enzymes) of up to 88 were obtained. Combinations of a ferulic acid esterase and xylanases resulted in synergy on all substrates, while a laccase and xylanases only acted synergistically on the more recalcitrant substrates. Synergy between different xylanases (glycoside hydrolase (GH) families 5 and 11) was observed particularly on oat hulls, producing a yield of 57%. The synergistic ability of the enzymes was found to be partly due to the increased enzyme stability when in combination with the substrates.
生物经济的成功建立取代了当前基于化石资源的经济，在很大程度上取决于我们降解顽固木质纤维素生物质的能力。本研究探讨了各种协同作用的酶在预处理后的农业副产物（玉米麸皮、燕麦壳、可溶和不可溶燕麦麸皮）上的应用潜力。协同度（用酶组合获得的寡糖产量除以用单个酶获得的产量之和）高达 88。在所有底物上，阿魏酸酯酶和木聚糖酶的组合都表现出协同作用，而漆酶和木聚糖酶仅在更顽固的底物上表现出协同作用。不同木聚糖酶（糖苷水解酶（GH）家族 5 和 11）之间的协同作用在燕麦壳上表现得尤为明显，产生了 57%的产量。发现酶的协同能力部分归因于与底物结合时酶稳定性的提高。
