Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University, Nan Bai Xiang Street, Wenzhou, 325006, Zhejiang, China.
Sci Rep. 2021 Oct 14;11(1):20492. doi: 10.1038/s41598-021-99724-9.
Recently some evidence has demonstrated the significance of PSMB8 in various malignancies. Nevertheless, PSMB8 (proteasome subunit beta 8), more familiar in the field of immunology contributing to the process of antigen presentation, is indeterminate in the role as a survival predictor of human pan-cancer. Besides, how PSMB8 interacts with immune cell infiltration in the tumor microenvironment requires further research. We then penetrated into the analysis of the PSMB8 expression profile among 33 types of cancer in the TCGA database. The results show that overexpression of PSMB8 was associated with poor clinical outcomes in overall survival (Sartorius et al. in Oncogene 35(22):2881-2892, 2016), disease-specific survival (DSS), disease-free interval (DFI), and progression-free interval (PFI) in most cancer varieties. In addition, there existed distinctly positive correlations between PSMB8 and immunity, reflected straightforwardly in the form of immune scores, tumor-infiltrating immune cells (TIICs) abundance, microsatellite instability, tumor mutation burden, and neoantigen level. Notably, specific markers of dendrite cells exhibited the tightest association with PSMB8 expression in terms of tumor-related immune infiltration patterns. Moreover, gene enrichment analysis showed that elevated PSMB8 expression was related to multiple immune-related pathways. We finally validated the PSMB8 expression in our local breast samples via quantitative PCR assays and concluded that PSMB8 appeared to perform well in predicting the survival outcome of BRCA patients. These findings elucidate the pivotal role of the antigen presentation-related gene PSMB8, which could potentially serve as a robust biomarker for prognosis determination in multiple cancers.
最近有证据表明 PSMEB8 在多种恶性肿瘤中具有重要意义。然而，PSMEB8（蛋白酶体亚基β8）在免疫学领域更为人熟知，它有助于抗原呈递过程，但在作为人类泛癌生存预测因子方面的作用尚不确定。此外，PSMEB8 如何与肿瘤微环境中的免疫细胞浸润相互作用还需要进一步研究。然后，我们深入分析了 TCGA 数据库中 33 种癌症中的 PSMEB8 表达谱。结果表明，PSMEB8 的过表达与总体生存（Sartorius 等人，Oncogene 35(22):2881-2892, 2016）、疾病特异性生存（DSS）、无病间隔（DFI）和无进展间隔（PFI）不良相关在大多数癌症中。此外，PSMEB8 与免疫之间存在明显的正相关，直接反映在免疫评分、肿瘤浸润免疫细胞（TIIC）丰度、微卫星不稳定性、肿瘤突变负担和新抗原水平上。值得注意的是，树突状细胞的特定标志物在肿瘤相关免疫浸润模式方面与 PSMEB8 表达的关联最为密切。此外，基因富集分析表明，PSMEB8 表达上调与多个免疫相关途径有关。我们最后通过定量 PCR 检测验证了我们本地的乳腺癌样本中的 PSMEB8 表达，并得出结论，PSMEB8 似乎在预测 BRCA 患者的生存结果方面表现良好。这些发现阐明了抗原呈递相关基因 PSMEB8 的关键作用，它可能成为多种癌症预后判断的强大生物标志物。
