Department of Urinary Surgery, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, People's Republic of China.
Department of Neurology, Central Hospital of Binzhou, Binzhou, Shandong, People's Republic of China.
Kaohsiung J Med Sci. 2020 Jul;36(7):494-500. doi: 10.1002/kjm2.12196. Epub 2020 Mar 3.
Abnormal expression of microRNAs (miRNAs) is frequently occurred in prostate cancer (PCa). This study was aimed to investigate the biological roles of miR-451a in PCa. Quantitative real-time PCR (qRT-PCR) and Western blot were employed to investigate the expression levels of miR-451a and proteasome (prosome, macropain) subunit, beta type, 8 (PSMB8) in PCa cell lines. Luciferase activity reporter assay was used to verify the connection between miR-451a and PSMB8. in vitro functional experiments were performed to measure the effects of miR-451a or PSMB8 on PCa cell proliferation, colony formation ability, cell invasion, and cell apoptosis. miR-451a expression was downregulated, whereas PSMB8 expression was upregulated in PCa cell lines. Luciferase activity reporter assay confirmed the direct connection between miR-451a and PSMB8. Overexpression of miR-451a inhibits PCa cell proliferation, colony formation, cell invasion and promotes cell apoptosis, while the overexpression of PSMB8 caused the opposite effects. Moreover, rescue experiments confirmed PSMB8 was a functional target of miR-451a. In conclusion, this study provides novel insights into the role of miR-451a in PCa, and the results demonstrated miR-451a could inhibit PCa progression by targeting PSMB8.
miRNAs（miRNA）的异常表达在前列腺癌（PCa）中经常发生。本研究旨在探讨miR-451a 在 PCa 中的生物学作用。采用定量实时 PCR（qRT-PCR）和 Western blot 检测 PCa 细胞系中 miR-451a 和蛋白酶体（蛋白酶体，巨蛋白酶）亚基β型8（PSMB8）的表达水平。荧光素酶活性报告基因检测用于验证 miR-451a 和 PSMB8 之间的联系。体外功能实验用于测量 miR-451a 或 PSMB8 对 PCa 细胞增殖、集落形成能力、细胞侵袭和细胞凋亡的影响。miR-451a 的表达在 PCa 细胞系中下调，而 PSMB8 的表达上调。荧光素酶活性报告基因检测证实了 miR-451a 和 PSMB8 之间的直接联系。过表达 miR-451a 抑制 PCa 细胞增殖、集落形成、细胞侵袭并促进细胞凋亡，而过表达 PSMB8 则产生相反的效果。此外，挽救实验证实 PSMB8 是 miR-451a 的功能靶标。综上所述，本研究为 miR-451a 在 PCa 中的作用提供了新的见解，结果表明 miR-451a 可以通过靶向 PSMB8 抑制 PCa 进展。
