Liu Nan, Li Xin, Wang Maofeng, Zhang Fengyu, Wang Chuandong, Zhang Kundi, Wang Hongwei, Xu Sujuan, Hu Wei, Gu Lichuan
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Front Microbiol. 2021 Sep 28;12:737458. doi: 10.3389/fmicb.2021.737458. eCollection 2021.
Billions of people suffer from dental caries every year in spite of the effort to reduce the prevalence over the past few decades. is the leading member of a specific group of cariogenic bacteria that cause dental caries. forms biofilm, which is highly resistant to harsh environment, host immunity, and antimicrobial treatments. In this study, we found that biofilm is highly resistant to both antimicrobial agents and lysozyme. DexA70, the truncated form of DexA (amino acids 100-732), a dextranase in , prevents biofilm formation and disassembles existing biofilms within minutes at nanomolar concentrations when supplied exogenously. DexA70 treatment markedly enhances biofilm sensitivity to antimicrobial agents and lysozyme, indicating its great potential in combating biofilm-related dental caries.
尽管在过去几十年里人们努力降低龋齿患病率，但每年仍有数十亿人患龋齿。[细菌名称]是导致龋齿的特定致龋菌群体中的主要成员。[细菌名称]形成生物膜，这种生物膜对恶劣环境、宿主免疫和抗菌治疗具有高度抗性。在本研究中，我们发现[细菌名称]生物膜对抗菌剂和溶菌酶都具有高度抗性。DexA70是DexA（氨基酸100 - 732）的截短形式，DexA是[细菌名称]中的一种葡聚糖酶，当外源提供时，它能在纳摩尔浓度下在几分钟内阻止[细菌名称]生物膜形成并分解现有的生物膜。DexA70处理显著增强了生物膜对抗菌剂和溶菌酶的敏感性，表明其在对抗与生物膜相关的龋齿方面具有巨大潜力。
