So Yeon Lee, Si Young Lee
Department of Oral Microbiology, College of Dentistry, Research Institute of Oral Science, Gangneung-Wonju National University.
Biocontrol Sci. 2019;24(1):13-21. doi: 10.4265/bio.24.13.
Although the most common bacteria in the supragingival plaque are Gram-positive streptococci, no extensive investigations have been conducted into the susceptibility of these species to chlorhexidine and cetylpyridinium chloride. Therefore, in this study, we investigated the susceptibility of 80 streptococcal strains in planktonic or biofilm states to these two antimicrobial agents. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the planktonic streptococci were measured using the microdilution method, as were the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) measured on streptococcal biofilms formed on 96-well plates. In all species, the MIC, MBC, MBIC, and MBEC values were higher for chlorhexidine than for cetylpyridinium chloride, with sensitivity values varying according to species. For chlorhexidine, the MIC, MBC, and MBIC values showed statistically significant differences among species. However, only MBEC values showed statistically significant differences for cetylpyridinium chloride. The MIC against Streptococcus mutans and the MBC against Streptococcus salivarius were significantly lower than those against the other species. With he exception of a few species, most of the bacterium susceptibility values were higher in the biofilm state than in the planktonic state.
虽然龈上菌斑中最常见的细菌是革兰氏阳性链球菌，但尚未对这些菌种对氯己定和西吡氯铵的敏感性进行广泛研究。因此，在本研究中，我们调查了80株处于浮游或生物膜状态的链球菌对这两种抗菌剂的敏感性。采用微量稀释法测定浮游链球菌的最低抑菌浓度（MIC）和最低杀菌浓度（MBC），以及在96孔板上形成的链球菌生物膜的最低生物膜抑制浓度（MBIC）和最低生物膜清除浓度（MBEC）。在所有菌种中，氯己定的MIC、MBC、MBIC和MBEC值均高于西吡氯铵，且敏感性值因菌种而异。对于氯己定，MIC、MBC和MBIC值在不同菌种间存在统计学显著差异。然而，对于西吡氯铵，只有MBEC值存在统计学显著差异。变形链球菌的MIC和唾液链球菌的MBC显著低于其他菌种。除少数菌种外，大多数细菌在生物膜状态下的敏感性值高于浮游状态。
