Essuman Edward Ken, Boakye Adjoa Agyemang, Tettey Clement Okraku, Hunkpe Gaston, Kortei Nii Korley, Kwansa-Bentum Henrietta, Waikhom Sayanika Davi, Aninagyei Enoch
Department of Nutrition and Dietetics, University of Health and Allied Sciences, Ho, Ghana.
Department of Biomedical Sciences, University of Health and Allied Sciences, Ho, Ghana.
Evid Based Complement Alternat Med. 2021 Oct 5;2021:7270250. doi: 10.1155/2021/7270250. eCollection 2021.
Microbial etiology of diarrhea is a significant cause of death, especially in children in developing countries. The presence of microbes that are resistant to current treatment options for diarrhea suggests the need to find newer antimicrobial agents for treatment. Therefore, this study focused on investigating the antimicrobial effect of some Ghanaian chewing sticks commonly used for oral hygiene, , and , against selected diarrhea-causing organisms. From the stem and bark of each plant, 70% methanolic extract was experimented on and species, namely, , , , attenuated, and for microbial susceptibility using the agar well diffusion method. Additionally, the antioxidant profile of the methanolic extracts were investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic) acid (ABTS) scavenging activities, and ferric-reducing antioxidant potential (FRAP) assays, while the total polyphenolic content was determined using the Folin-Ciocalteau reagent. and stem demonstrated the highest antimicrobial effect, inhibiting the growth of all test organisms. Additionally, the extracts demonstrated high antioxidant potential and were found to possess significant amounts of phenolic compounds. Therefore, methanolic extracts of and stem are promising candidates for the identification of safe novel compounds to mitigate diarrheal diseases.
腹泻的微生物病因是一个重要的死亡原因，尤其是在发展中国家的儿童中。对当前腹泻治疗方案具有抗性的微生物的存在表明需要寻找更新的抗菌药物用于治疗。因此，本研究着重调查一些常用于口腔卫生的加纳咀嚼棒对选定的腹泻致病生物的抗菌作用。从每种植物的茎和树皮中提取70%的甲醇提取物，使用琼脂扩散法对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌、伤寒沙门氏菌减毒株和志贺氏菌进行微生物敏感性实验。此外，使用2,2-二苯基-1-苦基肼基（DPPH）自由基、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）清除活性和铁还原抗氧化能力（FRAP）测定法研究甲醇提取物的抗氧化特性，同时使用福林-酚试剂测定总多酚含量。Ximenia americana和Khaya senegalensis茎表现出最高的抗菌效果，抑制了所有测试生物的生长。此外，提取物表现出高抗氧化潜力，并且发现含有大量酚类化合物。因此，Ximenia americana和Khaya senegalensis茎的甲醇提取物是鉴定安全新型化合物以减轻腹泻疾病的有前途的候选物。
