Shen Guoliang, Wu Luning, Jia Hangdong, Liu Junwei
General Surgery, Department of Hepatobiliary and Pancreatic Surgery and Minimal Invasive Surgery, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College Hangzhou 310014, Zhejiang, China.
Thyroid Gland Breast Surgery, Dongyang People's Hospital Jinhua 322100, Zhejiang, China.
Am J Transl Res. 2021 Sep 15;13(9):10288-10297. eCollection 2021.
To evaluate the feasibility of modified binding pancreatogastrostomy (MBPA) by comparing it with traditional pancreatogastrostomy (TPA) and to determine the surgical effects on the intestinal microecology.
The surgical effects on the intestinal microecology of female Bama minipigs (n = 20) were determined by measuring the expressions of the intestinal microbial proteins in the gastric juice, gastric mucosa, and feces before and after MBPA and TPA. We then constructed an integrated interaction network based on the metabolomics and 16S amplicon data, the microbiota, the metabolites, and the associated pathways.
The average time required for anastomosis was significantly lower after MBPA than after TPA, but the breaking force did not significantly differ between them. We identified 25 and 51 differentially expressed metabolites and microbiota, respectively. An interaction network was constructed using 16 metabolites (including pyruvic and lactic acids), 27 microbiota (including _UCG-00) and six pathways (including pyruvate metabolism).
Anastomosis might be achieved sooner and with less pancreatic leakage using MBPA compared with TPA. Pancreatogastrostomy inhibits activity, leading to increased expressions of pyruvic and lactic acids in the gut.
通过将改良捆绑式胰胃吻合术（MBPA）与传统胰胃吻合术（TPA）进行比较，评估其可行性，并确定其对肠道微生态的手术效果。
通过测量雌性巴马小型猪（n = 20）在MBPA和TPA前后胃液、胃黏膜和粪便中肠道微生物蛋白的表达，确定手术对肠道微生态的影响。然后基于代谢组学和16S扩增子数据、微生物群、代谢物及相关途径构建综合相互作用网络。
MBPA后吻合所需的平均时间显著低于TPA后，但两者之间的断裂力无显著差异。我们分别鉴定出25种和51种差异表达的代谢物和微生物群。使用16种代谢物（包括丙酮酸和乳酸）、27种微生物群（包括_UCG - 00）和6条途径（包括丙酮酸代谢）构建了一个相互作用网络。
与TPA相比，MBPA可能更快实现吻合且胰漏更少。胰胃吻合术抑制了活性，导致肠道中丙酮酸和乳酸的表达增加。
