The roles of the glucagon-like peptide-2 and the serum TGF-β1 levels in the intestinal barrier and immune functions in rats with obstructive jaundice.

OBJECTIVE

To determine the mechanisms by which glucagon-like peptide-2 (GLP-2) impacts the intestinal barrier function, the immune function, and the serum transforming growth factor-β1 (TGF-β1) levels in rats with obstructive jaundice.

METHODS

Overall, 72 SPF-grade healthy Wistar rats were randomly divided into 4 groups containing 18 rats each: the observation group (ligation of common bile duct, intraperitoneal GLP-2 injection), the control group (ligation of common bile duct, normal saline), the sham-operated group (common bile duct exposed without ligation, normal saline), and the blank group. The serum immune function and the TGF-β1 levels were measured on days 3, 7, and 14 after the intervention.

RESULTS

The body mass was determined to be significantly less in the control group than in the other three groups on day 14 after the intervention (P < 0.05). The TGF-β1, endotoxin, alanine aminotransferase (ALT), and bilirubin were expressed at significantly higher levels in the control group compared with the blank and sham-operated groups and were the highest at each time point, but the levels in the observation group were significantly decreased after the intervention (P < 0.05).

CONCLUSIONS

We found that GLP-2 can decrease the serum TGF-β1 levels, regulate the immune function, reduce the endotoxin and bilirubin, and protect the intestinal barrier function in rats with obstructive jaundice.