Department of General Surgery, Binzhou Medical University Hospital, No.661, Huanghe 2nd Road, Binzhou, 256603, Shandong, China.
Clinical Medicine Laboratory, Binzhou Medical University Hospital, Binzhou, 256603, Shandong, China.
Mol Biotechnol. 2024 Aug;66(8):1954-1960. doi: 10.1007/s12033-023-00829-5. Epub 2023 Jul 28.
Obstructive jaundice (OJ) can cause multiple pathophysiological consequences including intestinal barrier dysfunction. Omega-3 has been indicated to have a promising therapeutic effect on OJ. This study aimed to further investigate the functions of omega-3 on OJ-induced intestinal injury. A rat OJ model was established by bile duct ligation with or without omega-3 administration. ELISA was utilized for measuring serum levels of inflammatory cytokines. Hematoxylin-eosin staining and TUNEL staining were employed for detecting the morphological changes and cell apoptosis in rat intestine. Western blotting was utilized for evaluating expression of tight junction proteins in the intestinal tissues. Omgea-3 offset the reduction in body weight of OJ rats. Omega-3 alleviated inflammatory response, pathological damages and cell apoptosis in the intestine of OJ rats. Additionally, omega-3 enhanced levels of tight junction proteins in the intestinal tissues of OJ rats. Omega-3 ameliorates OJ-triggered impairment of intestinal barrier function in rats.
阻塞性黄疸（OJ）可引起多种病理生理后果，包括肠道屏障功能障碍。ω-3 已被证明对 OJ 具有有前途的治疗作用。本研究旨在进一步研究 ω-3 对 OJ 诱导的肠道损伤的作用。通过胆管结扎建立大鼠 OJ 模型，并给予或不给予 ω-3 治疗。ELISA 用于测量血清中炎症细胞因子的水平。苏木精-伊红染色和 TUNEL 染色用于检测大鼠肠道的形态变化和细胞凋亡。Western blot 用于评估肠道组织中紧密连接蛋白的表达。ω-3 减轻了 OJ 大鼠体重减轻。ω-3 减轻了 OJ 大鼠肠道的炎症反应、病理损伤和细胞凋亡。此外，ω-3 增强了 OJ 大鼠肠道组织中紧密连接蛋白的水平。ω-3 改善了 OJ 大鼠肠道屏障功能障碍。
