Hebei Sub-Center of National Maize Improvement Center, Key Laboratory Jointly Constructed by the Ministry of Education and Hebei Province, Key Laboratory for Crop Germplasm Resources of Hebei, North China Key Laboratory for Crop Germplasm Resources under the Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding, China.
College of Agronomy, Qingdao Agricultural University, Qingdao, China.
PLoS One. 2021 Oct 15;16(10):e0258327. doi: 10.1371/journal.pone.0258327. eCollection 2021.
General combining ability (GCA) is an important index for inbred lines breeding of maize. To identify the genetic loci of GCA and associated agronomic traits, an association analysis with 195 SSRs was made in phenotypic traits of 240 F1 derived from 120 elite inbred lines containing current breeding resources of maize crossed with 2 testers (Zheng58 and Chang7-2) in two places in 2018. All of the 20 association loci detected for grain yield (GY), plant height (PH), ear height (EH) and GCA for the three traits in two places could explain a phenotypic variation range of 7.31%-9.29%. Among the 20 association loci, 9 (7.31%-9.04%) were associated with GY, 4 (7.22%-8.91%) were related to GCA of GY, 1 (7.56%) was associated with PH, and 3 (7.53%-8.96%) were related to EH. In addition, 3 loci (9.14%-9.29%) were associated with GCA of PH whereas no locus was identified for GCA of EH. In the comparison of the association loci detected in Baoding and Handan, interestingly, one locus (7.69% and 8.11%) was identified in both environments and one locus (7.52% and 7.82%) was identified for yield and GCA of yield. Therefore, the identification of GY-, PH-, EH- and GCA-related association loci could not only provide references for high yield breeding of maize, but also help us comprehend the relationships among GY, agricultural traits and GCA.
一般配合力（GCA）是玉米自交系选育的重要指标。为了鉴定 GCA 和相关农艺性状的遗传位点，我们利用 195 个 SSR 对 240 个来自 120 个自交系的 F1 进行了表型分析，这些自交系包含了当前玉米的育种资源，与 2 个测验种（郑 58 和昌 7-2）在 2018 年的两个地点杂交。在两个地点检测到的 20 个与产量（GY）、株高（PH）、穗位高（EH）和三个性状的 GCA 相关的关联位点，都可以解释 7.31%-9.29%的表型变异范围。在这 20 个关联位点中，有 9 个（7.31%-9.04%）与 GY 相关，4 个（7.22%-8.91%）与 GY 的 GCA 相关，1 个（7.56%）与 PH 相关，3 个（7.53%-8.96%）与 EH 相关。此外，有 3 个位点（9.14%-9.29%）与 PH 的 GCA 相关，而没有与 EH 的 GCA 相关的位点。在保定和邯郸的关联位点比较中，有趣的是，有一个位点（7.69%和 8.11%）在两个环境中都被鉴定出来，有一个位点（7.52%和 7.82%）与产量和产量的 GCA 相关。因此，鉴定 GY、PH、EH 和 GCA 相关的关联位点不仅可以为玉米高产育种提供参考，还可以帮助我们理解 GY、农艺性状和 GCA 之间的关系。
