基于聚合酶链式反应的西尼罗河病毒下一代测序方案。
PCR-based next-generation West Nile virus sequencing protocols.
作者信息
Pappa Styliani, Chaintoutis Serafeim C, Dovas Chrysostomos I, Papa Anna
机构信息
National Reference Centre for Arboviruses, Department of Microbiology, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece.
Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece.
出版信息
Mol Cell Probes. 2021 Dec;60:101774. doi: 10.1016/j.mcp.2021.101774. Epub 2021 Oct 13.
The epidemiology of West Nile virus (WNV) is unpredictable and changing. Availability of whole genome sequences enables the detailed molecular epidemiology studies and the evaluation and design of diagnostic tools. In the present study we provide two PCR-based protocols which can be applied directly on biological samples from hosts infected by WNV strains belonging to lineage 1 or lineage 2. It was shown that the protocols worked successfully even on samples with relatively low viral load.
西尼罗河病毒（WNV）的流行病学具有不可预测性且不断变化。全基因组序列的可得性有助于开展详细的分子流行病学研究以及诊断工具的评估与设计。在本研究中，我们提供了两种基于聚合酶链反应（PCR）的方案，可直接应用于感染1型或2型WNV毒株的宿主的生物样本。结果表明，即使在病毒载量相对较低的样本上，这些方案也能成功发挥作用。
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