经恶臭假单胞菌预处理和工艺放大后，从石油炼油厂污泥中提高甲烷产量和去除烃类。
Enhanced methane production and hydrocarbon removal from petroleum refinery sludge after Pseudomonas putida pretreatment and process scale-up.
机构信息
Department of Civil Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Department of Civil Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
出版信息
Bioresour Technol. 2022 Jan;343:126127. doi: 10.1016/j.biortech.2021.126127. Epub 2021 Oct 12.
The influence of Pseudomonas putida 7525 strain on the pretreatment of petroleum refinery sludge was optimized at different dosages to maximize solubilization for improved biodegradability. Laccase-producing P. putida strain at a dosage of 10 CFU/mL resulted in 249% and 121.57% increments in soluble chemical oxygen demand and volatile fatty acids production respectively as compared to untreated within 6 days of incubation. 1L biochemical methane potential test conducted for optimization of different inoculum and pretreated substrate ratios (0.3, 0.4, 0.5, 0.7 and 1.0) revealed maximum methane augmentation (62%) and volatile solids degradation (66.7%) at ratio 0.5. Scaled-up study (20L) for ratio 0.5 resulted in 57.07% total petroleum hydrocarbon, 62.98% oil and grease and 91.9% phenol removal within 50 days of digestion of pretreated PS. Kinetic modelling of cumulative methane yield indicated that modified Gompertz model showed the best fit thereby, evincing the potency of bacterial species for bioremediation of PS.
优化了不同剂量的假单胞菌 7525 菌株对石油炼制污泥预处理的影响，以最大限度地提高溶解率，提高生物降解性。与未经处理的污泥相比，在孵育 6 天内，产漆酶的假单胞菌菌株的剂量为 10 CFU/mL 时，可溶性化学需氧量和挥发性脂肪酸的产量分别增加了 249%和 121.57%。为了优化不同接种物和预处理底物比例（0.3、0.4、0.5、0.7 和 1.0）进行了 1L 生化甲烷潜能测试，结果表明在比例为 0.5 时，甲烷的增加量最大（62%），挥发性固体的降解量最大（66.7%）。在比例为 0.5 的放大研究（20L）中，预处理 PS 消化 50 天内，总石油烃去除率为 57.07%，油和油脂去除率为 62.98%，苯酚去除率为 91.9%。累积甲烷产量的动力学模型表明，修正的 Gompertz 模型拟合度最好，从而证明了细菌物种对 PS 生物修复的潜力。
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