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深入了解高传染性冠状病毒的检测途径/生物传感器。

An Insight Into Detection Pathways/Biosensors of Highly Infectious Coronaviruses.

机构信息

Department of Genetic Engineering and Molecular Genetics, Zanjan University, Zanjan, Iran.

Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

出版信息

Mol Biotechnol. 2022 Apr;64(4):339-354. doi: 10.1007/s12033-021-00417-5. Epub 2021 Oct 16.

DOI:10.1007/s12033-021-00417-5
PMID:34655396
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8520350/
Abstract

The outbreak of COVID-19 pandemic and its consequences have inflicted a substantial damage on the world. In this study, it was attempted to review the recent coronaviruses appeared among the human being and their epidemic/pandemic spread throughout the world. Currently, there is an inevitable need for the establishment of a quick and easily available biosensor for tracing COVID-19 in all countries. It has been known that the incubation time of COVID-19 lasts about 14 days and 25% of the infected individuals are asymptomatic. To improve the ability to determine SARS-CoV-2 precisely and reduce the risk of eliciting false-negative results produced by mutating nature of coronaviruses, many researchers have established a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay using mismatch-tolerant molecular beacons as multiplex real-time RT-PCR to distinguish between pathogenic and non-pathogenic strains of coronaviruses. The possible mechanisms and pathways for the detection of coronaviruses by biosensors have been reviewed in this study.

摘要

COVID-19 大流行的爆发及其后果给世界造成了巨大的损失。在本研究中，我们试图回顾近年来在人类中出现的冠状病毒及其在世界范围内的流行/大流行传播。目前，各国都需要建立一种快速、易于获得的生物传感器，以追踪 COVID-19。已知 COVID-19 的潜伏期约为 14 天，25%的感染者无症状。为了提高准确确定 SARS-CoV-2 的能力，并降低冠状病毒变异导致假阴性结果的风险，许多研究人员已经建立了一种使用错配容忍分子信标的实时逆转录聚合酶链反应（RT-PCR）检测方法，用于多重实时 RT-PCR 区分冠状病毒的致病性和非致病性株。本研究综述了生物传感器检测冠状病毒的可能机制和途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/3716f4c71e04/12033_2021_417_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/c94814865b6e/12033_2021_417_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/7c15a2129c36/12033_2021_417_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/ac3c889d921e/12033_2021_417_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/98b20ecd9241/12033_2021_417_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/4b6d43bba8ba/12033_2021_417_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/3df4182d441b/12033_2021_417_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/26f2bbd7b4b6/12033_2021_417_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/1673903c8adf/12033_2021_417_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/3716f4c71e04/12033_2021_417_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/c94814865b6e/12033_2021_417_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/7c15a2129c36/12033_2021_417_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/ac3c889d921e/12033_2021_417_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/98b20ecd9241/12033_2021_417_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/4b6d43bba8ba/12033_2021_417_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/3df4182d441b/12033_2021_417_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/26f2bbd7b4b6/12033_2021_417_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/1673903c8adf/12033_2021_417_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4bc/8520350/3716f4c71e04/12033_2021_417_Fig9_HTML.jpg

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2
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Diseases. 2021 May 20;9(2):38. doi: 10.3390/diseases9020038.
3
CRISPR-Cas systems for diagnosing infectious diseases.CRISPR-Cas 系统在传染病诊断中的应用。
Methods. 2022 Jul;203:431-446. doi: 10.1016/j.ymeth.2021.04.007. Epub 2021 Apr 9.
4
Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets.SARS-CoV-2 RT-qPCR 引物探针组合的分析灵敏度和效率比较。
Nat Microbiol. 2020 Oct;5(10):1299-1305. doi: 10.1038/s41564-020-0761-6. Epub 2020 Jul 10.
5
A serological assay to detect SARS-CoV-2 seroconversion in humans.用于检测人类 SARS-CoV-2 血清转化的血清学检测方法。
Nat Med. 2020 Jul;26(7):1033-1036. doi: 10.1038/s41591-020-0913-5. Epub 2020 May 12.
6
Immunosensor Based on Antibody-Functionalized MoS for Rapid Detection of Avian Coronavirus on Cotton Thread.基于抗体功能化二硫化钼的免疫传感器用于棉线上禽冠状病毒的快速检测
IEEE Sens J. 2018 Apr 23;18(11):4358-4363. doi: 10.1109/JSEN.2018.2829084. eCollection 2018 Jun 1.
7
Isolation of SARS-CoV-2-related coronavirus from Malayan pangolins.从马来穿山甲中分离出 SARS-CoV-2 相关冠状病毒。
Nature. 2020 Jul;583(7815):286-289. doi: 10.1038/s41586-020-2313-x. Epub 2020 May 7.
8
A human monoclonal antibody blocking SARS-CoV-2 infection.一种人源单克隆抗体阻断 SARS-CoV-2 感染。
Nat Commun. 2020 May 4;11(1):2251. doi: 10.1038/s41467-020-16256-y.
9
Quantitative and sensitive detection of SARS coronavirus nucleocapsid protein using quantum dots-conjugated RNA aptamer on chip.基于芯片上量子点偶联RNA适配体的严重急性呼吸综合征冠状病毒核衣壳蛋白的定量灵敏检测
J Chem Technol Biotechnol. 2011 Dec;86(12):1475-1479. doi: 10.1002/jctb.2721. Epub 2011 Oct 3.
10
Connecting clusters of COVID-19: an epidemiological and serological investigation.连接 COVID-19 集群：一项流行病学和血清学调查。
Lancet Infect Dis. 2020 Jul;20(7):809-815. doi: 10.1016/S1473-3099(20)30273-5. Epub 2020 Apr 21.