Profiling of the spatiotemporal distribution, risks, and prioritization of antibiotics in the waters of Laizhou Bay, northern China.

We investigated the spatiotemporal distributions, risks, and prioritization of 15 widely used antibiotics in Laizhou Bay (LZB). Water samples (145) were collected from LZB and its estuaries and analyzed. Twelve antibiotics, with total concentrations of 241-1450 and 69-289 ng L in estuarine water and seawater, respectively, were detected, with the contributions of norfloxacin, ciprofloxacin, and amoxicillin exceeding 70%. Amoxicillin was firstly determined, which contributed to 20% and 46% of the total antibiotics during summer and spring, respectively. Higher antibiotic concentrations were observed in the sea located adjacent to aquaculture bases and the Yellow River Estuary, which are significantly influenced by mariculture and riverine inputs, respectively. Veterinary antibiotics showed higher total concentrations in summer compared to spring, indicating a higher degree of their usage in mariculture in summer. The antibiotic mixtures posed high risk to algae and low to medium risks to crustaceans and fish. Amoxicillin and norfloxacin were identified as high-risk pollutants. Additionally, amoxicillin and ciprofloxacin showed medium to high resistance development risks. Previous studies on antibiotics in the LZB did not determined amoxicillin and thus underestimated antibiotic contamination, ecological risk, and resistance development risk. Amoxicillin, norfloxacin, and ciprofloxacin should be prioritized in risk management.