Chair of Food Science, Institute of Nutritional Science, and Interdisciplinary Research Center, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Chair of Food Science, Institute of Nutritional Science, and Interdisciplinary Research Center, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Nov 1;1184:122956. doi: 10.1016/j.jchromb.2021.122956. Epub 2021 Oct 2.
A rapid and straightforward approach was developed for screening the acetyl- and butyrylcholinesterase (ChE) inhibitory activity of 44 Iranian medicinal plant extracts at laboratory scale. After a fast ChE inhibitory pre-testing of samples applied as band pattern, 40 out of the 44 Iranian medicinal plant extracts were selected. These were adjusted in the application volume depending on their inhibition activity, applied on both plate sides and simultaneously developed in a horizontal developing chamber. Different mobile phases were studied to achieve maximum separation of ChE inhibitors and minimum co-elution with matrix. Contrary to immersion, the piezoelectric spraying reduced the consumption of assay solutions, prevented zone tailing, zone shift and cross-contamination, and homogeneously covered the entire plate surface with the assay solutions. The ChE inhibitors of the six most bioactive plant extracts were tentatively assigned by high-resolution mass spectrometry in combination with the spectral and chromatographic information obtained.
建立了一种快速而直接的方法，用于在实验室规模上筛选 44 种伊朗药用植物提取物对乙酰胆碱酯酶和丁酰胆碱酯酶（ChE）的抑制活性。在快速进行 ChE 抑制预测试后，将作为条带模式应用的样品中，有 40 种伊朗药用植物提取物被选中。根据其抑制活性调整应用体积，应用于板的两侧，并在水平展开室中同时展开。研究了不同的流动相以实现 ChE 抑制剂的最大分离和与基质的最小共洗脱。与浸泡法相比，压电喷雾减少了分析溶液的消耗，防止了区带拖尾、区带移位和交叉污染，并使分析溶液均匀地覆盖整个板面。通过高分辨率质谱与获得的光谱和色谱信息相结合，对六种最具生物活性的植物提取物的 ChE 抑制剂进行了初步分配。
