State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Zhengzhou Research Base, School of Agricultural Sciences, Zhengzhou University, Key Laboratory for Cotton Genetic Improvement, MOA, Anyang, Henan 455000, China; Engineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, 830052 Urumqi, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Zhengzhou Research Base, School of Agricultural Sciences, Zhengzhou University, Key Laboratory for Cotton Genetic Improvement, MOA, Anyang, Henan 455000, China.
Int J Biol Macromol. 2021 Dec 1;192:1058-1074. doi: 10.1016/j.ijbiomac.2021.10.038. Epub 2021 Oct 14.
Cotton is the most important crop for the production of natural fibres used in the textile industry. High salinity, drought, cold and high temperature represent serious abiotic stresses, which seriously threaten cotton production. Phospholipase A has an irreplaceable role in lipid signal transmission, growth and development and stress events. Phospholipase A can be divided into three families: PLA1, PLA2 and pPLA. Among them, the PLA1 family is rarely studied in plants. In order to study the potential functions of the PLA1 family in cotton, the bioinformatics analysis of the PLA1 family was correlated with cotton adversity, and tissue-specific analysis was performed. Explore the structure-function relationship of PLA1 members. It is found that the expression of GbPLA1-32 gene is affected by a variety of environmental stimuli, indicating that it plays a very important role in stress and hormone response, and closely associates the cotton adversity with this family. Through further functional verification, we found that virus-induced GbPLA1-32 gene silencing (VIGS) caused Gossypium barbadense to be sensitive to salt stress. This research provides an important basis for further research on the molecular mechanism of cotton resistance to abiotic stress.
棉花是用于纺织工业的天然纤维生产中最重要的作物。高盐度、干旱、寒冷和高温代表着严重的非生物胁迫，严重威胁着棉花的生产。磷脂酶 A 在脂质信号转导、生长发育和应激事件中具有不可替代的作用。磷脂酶 A 可分为 PLA1、PLA2 和 pPLA 三种家族。其中，PLA1 家族在植物中研究较少。为了研究 PLA1 家族在棉花中的潜在功能，对 PLA1 家族进行了生物信息学分析，并与棉花逆境相关联，进行了组织特异性分析。探讨 PLA1 成员的结构-功能关系。发现 GbPLA1-32 基因的表达受多种环境刺激的影响，表明其在胁迫和激素反应中发挥着非常重要的作用，并将棉花逆境与该家族紧密联系在一起。通过进一步的功能验证，我们发现病毒诱导的 GbPLA1-32 基因沉默（VIGS）导致 Gossypium barbadense 对盐胁迫敏感。这项研究为进一步研究棉花抗非生物胁迫的分子机制提供了重要依据。
