Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain.
Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain.
Fish Shellfish Immunol. 2021 Dec;119:623-634. doi: 10.1016/j.fsi.2021.10.009. Epub 2021 Oct 14.
Although inflammation is a well-characterized process in mammals, few studies have dealt with the mechanisms involved in this process in fish. The present study evaluated the expression of inflammation-related genes in the skin of fish injected with carrageenin, which has previously been used in inflammatory models in mammals. In our case, fish were injected subcutaneously with PBS (as control) or carrageenin (1%), and skin samples from the injection site were collected 1.5, 3 and 6 h post-injection. The gene expression of inflammatory markers (csfr1, mhc-ii and phox40), several pro-inflammatory cytokines (il1b, tnfa, il6, il8 and il18) and other molecules related (such as myd88 and c-rel) were up-regulated at 1.5 and 3 h in fish injected with carrageenin compared with control levels. By contrast, the gene expression of anti-inflammatory molecules (nlrx1, nlrc5 isoform 1, ctsd and ctss) was down-regulated in fish injected with carrageenin and sampled 3 h post injection, again compared to the gene expression in control fish. According to our results, carrageenin can be considered not only a good stimulator to study skin inflammation in gilthead seabream but also this method might be use to study the modulation of fish inflammatory process caused by internal or external factors.
虽然炎症是哺乳动物中一个特征明确的过程，但很少有研究涉及鱼类中涉及该过程的机制。本研究评估了在角叉菜胶（先前已用于哺乳动物炎症模型）注射后的鱼类皮肤中与炎症相关的基因的表达。在我们的案例中，鱼被皮下注射 PBS（作为对照）或角叉菜胶（1%），并在注射后 1.5、3 和 6 小时收集注射部位的皮肤样本。与对照水平相比，在注射角叉菜胶的鱼类中，炎症标志物（csfr1、mhc-ii 和 phox40）、几种促炎细胞因子（il1b、tnfa、il6、il8 和 il18）和其他相关分子（如 myd88 和 c-rel）的基因表达在 1.5 和 3 小时时上调。相比之下，在注射角叉菜胶并在 3 小时取样的鱼类中，抗炎分子（nlrx1、nlrc5 同种型 1、ctsd 和 ctss）的基因表达下调，再次与对照鱼类中的基因表达相比。根据我们的结果，角叉菜胶不仅可以被认为是研究金头鲷皮肤炎症的良好刺激物，而且这种方法也可以用于研究由内部或外部因素引起的鱼类炎症过程的调节。
