Sánchez-Bonet Desirée, García-Oms Samanta, Belda-Antolí Mariola, Padrón-Sanz Carolina, Lloris-Carsi José Miguel, Cejalvo-Lapeña Dolores
Escuela de Doctorado, Universidad Católica de Valencia "San Vicente Mártir", C/Guillem de Castro, 65, 46008 Valencia, Spain.
Centro de Investigación Traslacional San Alberto Magno, Universidad Católica de Valencia "San Vicente Mártir", C/Quevedo, 2, 46001 Valencia, Spain.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Nov 1;1184:122978. doi: 10.1016/j.jchromb.2021.122978. Epub 2021 Oct 5.
Significant quantities of bioactive compounds have been found in the chemical composition of seaweeds. This source of natural antioxidants such as polyphenols appears to attenuate lipid peroxidation caused by oxidative stress, preventing the harmful effects of a number of injuries including ischemia-reperfusion (I/R). Conventional extraction (CE) has been used for years as a traditional method for obtaining bioactive components from seaweeds. However, recent studies highlight ultrasonic-assisted extraction (UAE) as an alternative and more eco-friendly technique. Therefore, the two methods were optimised and compared to obtain a Fucus vesiculosus extract (FVE) with high antioxidant activity and polyphenol content. The highest antioxidant activity was obtained after 1 h at 25 °C for conventional extraction, and after 5 min at 35 °C for ultrasonic-assisted extraction. Higher concentrations of polyphenols were obtained with the optimal conditions in conventional extraction (13.61 mg PGE/g seaweed), but no significant differences were observed between the antioxidant activity obtained with UAE (89.33%) and CE (89.74%). The characterization of the polyphenols present in both optimised extracts was carried out and compared with reverse-phase high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). The following compounds were identified: phloroglucinol, gallic acid, catechin, vanillic acid, epicatechin, protocatechuic acid, rutin, gentisic acid, chlorogenic acid, caffeic acid, coumaric acid and ferulic acid. RP-HPLC-DAD results also showed higher concentrations of polyphenols in optimised extracts with CE. Consequently, CE was found to be more effective than UAE in providing extracts with higher concentrations of polyphenols, but UAE constitutes an efficient and more eco-friendly methodology for obtaining a FVE with the highest antioxidant activity.
在海藻的化学成分中发现了大量生物活性化合物。这种天然抗氧化剂来源，如多酚，似乎可以减轻氧化应激引起的脂质过氧化，预防包括缺血再灌注（I/R）在内的多种损伤的有害影响。传统提取法（CE）多年来一直作为从海藻中获取生物活性成分的传统方法。然而，最近的研究强调超声辅助提取法（UAE）是一种替代且更环保的技术。因此，对这两种方法进行了优化和比较，以获得具有高抗氧化活性和多酚含量的墨角藻提取物（FVE）。传统提取在25℃下1小时后获得最高抗氧化活性，超声辅助提取在35℃下5分钟后获得最高抗氧化活性。在传统提取的最佳条件下获得了更高浓度的多酚（13.61 mg PGE/g海藻），但超声辅助提取（89.33%）和传统提取（89.74%）获得的抗氧化活性之间未观察到显著差异。对两种优化提取物中存在的多酚进行了表征，并与配备二极管阵列检测器的反相高效液相色谱法（HPLC-DAD）进行了比较。鉴定出了以下化合物：间苯三酚、没食子酸、儿茶素、香草酸、表儿茶素、原儿茶酸、芦丁、龙胆酸、绿原酸、咖啡酸、香豆酸和阿魏酸。RP-HPLC-DAD结果还显示，优化后的传统提取物中多酚浓度更高。因此，发现传统提取在提供更高浓度多酚的提取物方面比超声辅助提取更有效，但超声辅助提取是获得具有最高抗氧化活性的墨角藻提取物的一种高效且更环保的方法。
