超声探头提取方法的优化及[具体对象]酚类成分的新见解

Optimization of a Sonotrode Extraction Method and New Insight of Phenolic Composition of .

作者信息

Gil-Martínez Lidia, Santos-Mejías Alejandro, De la Torre-Ramírez José Manuel, Baños Alberto, Verardo Vito, Gómez-Caravaca Ana M

机构信息

Department of Chemistry and Natural Products, DMC Research Center, Camino de Jayena s/n, 18620 Alhendín, Spain.

EpiChron Research Group, Aragon Health Sciences Institute (IACS), IIS Aragón, Miguel Servet University Hospital, 50009 Zaragoza, Spain.

出版信息

Mar Drugs. 2025 Jan 14;23(1):40. doi: 10.3390/md23010040.

The optimization of bioactive compound extraction from using ultrasound-assisted extraction (UAE) via sonotrode was investigated to maximize phenolic recovery and antioxidant activity while promoting a sustainable process. Optimal conditions (40% / ethanol in water, 38 min, 36% amplitude) were selected to maximize phenolic recovery while considering environmental and energy sustainability by optimizing extraction efficiency and minimizing solvent and energy usage. HPLC-ESI-QTOF-MS analysis tentatively identified 25 phenolic compounds, including sulfated phenolic acids, phlorotannins, flavonoids, and halophenols, with some reported for the first time in , underscoring the complexity of this alga's metabolome. The antioxidant activity of the optimized extract was evaluated through FRAP (143.7 µmol TE/g), DPPH (EC 105.6 µg/mL), and TEAC (189.1 µmol Trolox/g) assays. The optimized process highlights as a valuable source of natural antioxidants, with potential applications in biotechnology, cosmetics, and food industries.

研究了通过超声探头利用超声辅助提取（UAE）从[具体藻类名称未给出]中提取生物活性化合物的优化方法，以在促进可持续过程的同时，最大限度地提高酚类物质的回收率和抗氧化活性。选择了最佳条件（40%乙醇/水、38分钟、36%振幅），通过优化提取效率并最小化溶剂和能源使用，在考虑环境和能源可持续性的同时最大限度地提高酚类物质的回收率。HPLC-ESI-QTOF-MS分析初步鉴定出25种酚类化合物，包括硫酸化酚酸、褐藻多酚、黄酮类化合物和卤代酚，其中一些在[具体藻类名称未给出]中是首次报道，这突出了这种藻类代谢组的复杂性。通过FRAP（143.7 μmol TE/g）、DPPH（EC 105.6 μg/mL）和TEAC（189.1 μmol Trolox/g）测定评估了优化提取物的抗氧化活性。优化后的工艺突出了[具体藻类名称未给出]作为天然抗氧化剂的宝贵来源，在生物技术、化妆品和食品工业中具有潜在应用。