Research Group for Bioactives-Analysis and Application, National Food Institute, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
Department of Biotechnology and Biomedicine, DTU Bioengineering, Technical University of Denmark, Søltofts Plads 221, 2800 Kongens Lyngby, Denmark.
Mar Drugs. 2022 Apr 12;20(4):263. doi: 10.3390/md20040263.
This study was aimed at investigating the effect of low polarity water (LPW) on the extraction of bioactive compounds from and to examine the influence of temperature on the extraction yield, total phenolic content, crude alginate, fucoidan content, and antioxidant activity. The extractions were performed at the temperature range of 120-200 °C with 10 °C increments, and the extraction yield increased linearly with the increasing extraction temperature, with the highest yields at 170-200 °C and with the maximum extraction yield (25.99 ± 2.22%) at 190 °C. The total phenolic content also increased with increasing temperature. The extracts showed a high antioxidant activity, measured with DPPH (2,2-Diphenyl-1-picrylhydrazyl) radicals scavenging and metal-chelating activities of 0.14 mg/mL and 1.39 mg/mL, respectively. The highest yield of alginate and crude fucoidan were found at 140 °C and 160 °C, respectively. The alginate and crude fucoidan contents of the extract were 2.13% and 22.3%, respectively. This study showed that the extraction of bioactive compounds from seaweed could be selectively maximized by controlling the polarity of an environmentally friendly solvent.
本研究旨在探究低极性水（LPW）对从海草中提取生物活性化合物的影响，并研究温度对提取产率、总酚含量、粗褐藻胶、褐藻糖胶含量和抗氧化活性的影响。提取在 120-200°C 的温度范围内进行，每 10°C 增加一次，提取产率随提取温度的升高呈线性增加，在 170-200°C 时产率最高，在 190°C 时达到最大提取产率（25.99±2.22%）。总酚含量也随温度的升高而增加。提取物表现出高的抗氧化活性，用 DPPH（2,2-二苯基-1-苦基肼）自由基清除和金属螯合活性分别测量为 0.14mg/mL 和 1.39mg/mL。褐藻胶和粗褐藻糖胶的最高产率分别在 140°C 和 160°C 时达到。提取物中的褐藻胶和粗褐藻糖胶含量分别为 2.13%和 22.3%。本研究表明，通过控制环境友好型溶剂的极性，可以有选择性地最大化从海藻中提取生物活性化合物。
