Chen Eric H-L, Weng Cheng-Wei, Li Yi-Min, Wu Ming-Chin, Yang Chien-Chih, Lee Kung-Ta, Chen Rita P-Y, Cheng Chiu-Ping
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
Institute of Plant Biology, National Taiwan University, Taipei, Taiwan.
Front Plant Sci. 2021 Sep 30;12:753217. doi: 10.3389/fpls.2021.753217. eCollection 2021.
Plant diseases are important issues in agriculture, and the development of effective and environment-friendly means of disease control is crucial and highly desired. Antimicrobial peptides (AMPs) are known as potential alternatives to chemical pesticides because of their potent broad-spectrum antimicrobial activity and because they have no risk, or have only a low risk, of developing chemical-resistant pathogens. In this study, we designed a series of amphipathic helical peptides with different spatial distributions of positive charges and found that the peptides that had a special sequence pattern "BBHBBHHBBH" ("B" for basic residue and "H" for hydrophobic residue) displayed excellent bactericidal and fungicidal activities in a wide range of economically important plant pathogens. The peptides with higher helical propensity had lower antimicrobial activity. When we modified the peptides with a long acyl chain at their N-terminus, their plant protection effect improved. Our application of the fatty acyl-modified peptides on the leaves of tomato and Arabidopsis plants lessened the infection caused by subsp. and . Our study provides important insights on the development of more potent novel AMPs for plant protection.
植物病害是农业中的重要问题，开发有效且环境友好的病害控制手段至关重要且备受期待。抗菌肽（AMPs）因其强大的广谱抗菌活性以及不存在或仅有低风险产生抗化学病原体的特性，被认为是化学农药的潜在替代品。在本研究中，我们设计了一系列具有不同正电荷空间分布的两亲性螺旋肽，发现具有特殊序列模式“BBHBBHHBBH”（“B”代表碱性残基，“H”代表疏水残基）的肽在多种具有经济重要性的植物病原体中表现出优异的杀菌和杀真菌活性。螺旋倾向较高的肽具有较低的抗菌活性。当我们在肽的N端用长酰链进行修饰时，它们的植物保护效果得到改善。我们将脂肪酰基修饰的肽应用于番茄和拟南芥植物的叶片上，减轻了由[具体亚种名称1]和[具体亚种名称2]引起的感染。我们的研究为开发更有效的新型用于植物保护的抗菌肽提供了重要见解。
