Levy D, Larner A, Chaudhuri A, Babiss L E, Darnell J E
Proc Natl Acad Sci U S A. 1986 Dec;83(23):8929-33. doi: 10.1073/pnas.83.23.8929.
A human gene (termed ISG-54K) that is induced from near undetectable levels to high transcriptional activity by alpha- and beta-interferons has been cloned. The genomic structure and nucleotide sequence of the coding region were determined and the RNA initiation site was identified. The 5' portion of the gene was fused with a heterologous gene lacking an active promoter in recombinant plasmid and adenoviral vectors. These fusion genes were used to assess the activity of the ISG-54K promoter in response to interferon. RNA was formed in HeLa cells from recombinant plasmids only in response to interferon. Furthermore, in human diploid fibroblasts, infection with the recombinant adenovirus vector resulted in a 50-fold increase in specific RNA in response to interferon, followed by a subsequent decrease, imitating the natural regulated transcriptional cycle of the endogenous gene.
一种人类基因（称为ISG - 54K）已被克隆，该基因可被α和β干扰素从几乎不可检测的水平诱导至高转录活性。确定了编码区的基因组结构和核苷酸序列，并鉴定了RNA起始位点。该基因的5'部分与重组质粒和腺病毒载体中缺乏活性启动子的异源基因融合。这些融合基因用于评估ISG - 54K启动子对干扰素的反应活性。仅在干扰素存在时，HeLa细胞中重组质粒才能形成RNA。此外，在人二倍体成纤维细胞中，重组腺病毒载体感染导致对干扰素产生的特异性RNA增加50倍，随后下降，模仿了内源基因自然调节的转录周期。
