Alix S, Lebrun L, Marais S, Philippe E, Bourmaud A, Baley C, Morvan C
Université de Rouen, Laboratoire PBS, UMR 6270 CNRS, FR 3038, 76821 Mont-Saint-Aignan Cedex, France.
Dehondt Technologies, ZI Rue Denis Papin. 76330 Notre Dame de Gravenchon, France.
Carbohydr Polym. 2012 Jan 4;87(1):177-185. doi: 10.1016/j.carbpol.2011.07.035. Epub 2011 Aug 2.
This study is focused on enzymatically upgrading the functional properties of flax fibres. Green flax fibres were treated with a polygalacturonase and a pectate lyase (PaL) and their properties were compared with dew-retted fibres. Morphological observations, vapour-sorption analyses and mechanical measurements showed that PaL-treatment was able not only to mime retting in terms of bundle division, but also to improve the mechanical properties of technical fibres. Conversely, these properties were shifted down after the polygalacturonase treatment, mainly due to the presence of contaminating glycanases. At the level of the elementary fibres, nanoindentation data indicated the highest stiffness of the secondary wall for PaL-treated fibres. The tensile properties exhibited equal, but moderate values of the Young's modulus (∼37±14GPa) and breaking strength (∼650±300MPa) for retted and PaL-treated fibres; we hypothesize an impact of the growth conditions on the fibre chemical structure with an excess of matrix pectins compared to the amount of glucomannan coating the cellulose microfibrils.
本研究聚焦于通过酶法提升亚麻纤维的功能特性。用聚半乳糖醛酸酶和果胶酸裂解酶（PaL）处理绿色亚麻纤维，并将其性能与雨露沤麻纤维进行比较。形态学观察、蒸汽吸附分析和力学测量表明，PaL处理不仅能够在束分裂方面模拟沤麻过程，还能改善工业用纤维的力学性能。相反，聚半乳糖醛酸酶处理后这些性能下降，主要是由于存在污染性聚糖酶。在单纤维水平上，纳米压痕数据表明PaL处理的纤维次生壁具有最高的硬度。沤麻纤维和PaL处理的纤维的拉伸性能表现出相等但适中的杨氏模量值（约37±14GPa）和断裂强度值（约650±300MPa）；我们推测生长条件对纤维化学结构有影响，与包覆纤维素微纤丝的葡甘露聚糖量相比，基质果胶过量。
