竹荪子实体中水溶性多糖的结构分析及其体内抗氧化活性
Structural analysis of water-soluble polysaccharides in the fruiting body of Dictyophora indusiata and their in vivo antioxidant activities.
作者信息
Hua Yanglin, Yang Bao, Tang Jian, Ma Zhonghua, Gao Qing, Zhao Mouming
机构信息
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; Infinitus (China) Company Ltd., Guangzhou 510665, China.
Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
出版信息
Carbohydr Polym. 2012 Jan 4;87(1):343-347. doi: 10.1016/j.carbpol.2011.07.056. Epub 2011 Aug 3.
The water-soluble Dictyophora indusiata polysaccharides (DIP) were extracted from the fruiting body of D. indusiata. The structural features of purified DIPs I and II were investigated. The results indicated that DIP I was composed of glucose (Glc) and mannose (Man) with molecular weight of 2100kDa, while DIP II comprised of xylose (Xyl), galactose (Gal), glucose (Glc) and Man with molecular weight of 18.16kDa. The glycosidic linkage of DIP I was composed of →1)-Glc-(6→: →1)-Man-(3,6→ with the ratio of 5.6:1.0, while DIP II was composed of →1)-Glc-(6→: →1)-Man-(3,6→: →1)-Xyl-(5→: →1)-Gal-(3→: →1)-Gal-(6→: with the ratio of 4.9: 15.5: 7.8: 1.0: 5.7. DIP significantly (P<0.05) decreased the malondialdehyde (MDA), lipofuscin levels and increased the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities of mice. The strong in vivo antioxidant activity indicated DIP had great potential as functional food.
水溶性竹荪多糖（DIP）从竹荪子实体中提取。对纯化后的DIP I和DIP II的结构特征进行了研究。结果表明，DIP I由葡萄糖（Glc）和甘露糖（Man）组成，分子量为2100 kDa，而DIP II由木糖（Xyl）、半乳糖（Gal）、葡萄糖（Glc）和甘露糖组成，分子量为18.16 kDa。DIP I的糖苷键由→1)-Glc-(6→: →1)-Man-(3,6→组成，比例为5.6:1.0，而DIP II由→1)-Glc-(6→: →1)-Man-(3,6→: →1)-Xyl-(5→: →1)-Gal-(3→: →1)-Gal-(6→:组成，比例为4.9: 15.5: 7.8: 1.0: 5.7。DIP显著（P<0.05）降低了小鼠的丙二醛（MDA）、脂褐素水平，并提高了超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性。其强大的体内抗氧化活性表明DIP作为功能性食品具有巨大潜力。
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