Purification and identification of one glucan from golden oyster mushroom (Pleurotus citrinopileatus (Fr.) Singer).

One water-soluble glucan (PCP-W1) was purified from the crude polysaccharide of Pleurotus citrinopileatus by chromatography on DEAE Sepharose Fast Flow and Sephadex G-200 column, and PCP-W1 (Mw=45kDa), was predominantly composed of Glc. Partial acid hydrolysis, Smith degradation-periodate oxidation, methylation analysis, Fourier transform infrared spectroscopy (FTIR), gas chromatography-mass spectrometry (GC-MS) analysis and nuclear magnetic resonance spectroscopy (NMR) experiments were conducted to elucidate its structure. The results indicated that PCP-W1 had a glucan backbone consisting of (1→6)-linked-β-d-glucopyranosyl residues, which were branched at O-3 position of the backbone with (1→3)-linked-β-d-glucopyranosyl and non-reducing end 1-β-d-glucopyranosyl residues. The repeating unit of the polysaccharide was established as.