来自毛壳菌属的一种新型β-甘露聚糖酶的高水平表达，该酶表现出高效的甘露聚糖水解活性。
High level expression of a novel β-mannanase from Chaetomium sp. exhibiting efficient mannan hydrolysis.
作者信息
Katrolia Priti, Zhou Peng, Zhang Pan, Yan Qiaojuan, Li Yinan, Jiang Zhengqiang, Xu Haibo
机构信息
Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing 100083, China.
出版信息
Carbohydr Polym. 2012 Jan 4;87(1):480-490. doi: 10.1016/j.carbpol.2011.08.008. Epub 2011 Aug 11.
A novel β-mannanase gene (CsMan5A) was cloned from Chaetomium sp. CQ31 and expressed in Pichia pastoris. It had an open reading frame of 1251bp encoding 416 amino acids and contained two introns. The deduced amino acid sequence shared the highest similarity (73%) with the β-mannanase from Emericella nidulans and belongs to glycosyl hydrolase family 5. The recombinant β-mannanase (CsMan5A) was secreted at extremely high levels of 50,030UmL and 6.1mgmL in high cell density fermentor. The purified enzyme was optimally active at pH 5.0 and 65°C and displayed broad pH stability (pH 5.0-11.0) and exhibited specificity towards locust bean gum (K=3.1mgmL), guar gum (K=9.3mgmL) and konjac powder (K=10.5mgmL). It efficiently degraded mannan polysaccharides into mannose and mannooligosacccharides, and also hydrolyzed mannotriose and mannotetraose. These properties make CsMan5A highly useful in food, feed and paper/pulp industries.
从嗜热栖热放线菌CQ31中克隆出一个新的β-甘露聚糖酶基因（CsMan5A），并在毕赤酵母中表达。它有一个1251bp的开放阅读框，编码416个氨基酸，包含两个内含子。推导的氨基酸序列与构巢曲霉的β-甘露聚糖酶相似度最高（73%），属于糖基水解酶家族5。重组β-甘露聚糖酶（CsMan5A）在高细胞密度发酵罐中以50,030U/mL和6.1mg/mL的极高水平分泌。纯化后的酶在pH 5.0和65°C时活性最佳，具有较宽的pH稳定性（pH 5.0 - 11.0），对刺槐豆胶（K = 3.1mg/mL）、瓜尔豆胶（K = 9.3mg/mL）和魔芋粉（K = 10.5mg/mL）具有特异性。它能有效地将甘露聚糖多糖降解为甘露糖和甘露寡糖，还能水解甘露三糖和甘露四糖。这些特性使得CsMan5A在食品、饲料和造纸/纸浆工业中非常有用。
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