Yu Shu-Huei, Wu Shao-Jung, Tang Deh-Wei, Ho Yi-Cheng, Mi Fwu-Long, Kuo Tzu-Hung, Sung Hsing-Wen
Department of Polymer Materials, Vanung University, Chung-Li, Taiwan, ROC.
Department of Chemical Engineering, MingChi University of Technology, Taipei, Taiwan, ROC.
Carbohydr Polym. 2012 Jan 4;87(1):531-536. doi: 10.1016/j.carbpol.2011.08.015. Epub 2011 Aug 11.
The development of stimuli-responsive materials in response to the molecules involved in biological processes has gained increased attentions. In this work, carboxymethyl chitosan (CM-chitosan) and poly(γ-glutamic acid) (pGlu) were reacted with a naturally occurring compound, genipin, leading to the formation of genipin-crosslinked CM-chitosan/pGlu conjugates with fluorescence emissions. The genipin-conjugated polymers were sensitive to the oxidation product of glucose, gluconic acid and hydrogen peroxide (HO). Fluorescence emissions of the polymers were quenched by gluconic acid and HO. An increase in the hydrodynamic diameter together with the quenching of fluorescence indicated that the genipin-conjugated polymers were self-aggregated into nanoparticles, in response to the stimulus of gluconic acid (but not for HO). Bovine serum albumin (BSA) could be loaded in the self-aggregated nanoparticles, and the incorporated BSA slowly released from the nanoparticles under hyper-gluconic acid conditions. This material is hence proposed as a stimuli-responsive material for optical sensing and protein delivery purposes.
响应生物过程中所涉及分子的刺激响应材料的开发已受到越来越多的关注。在这项工作中，羧甲基壳聚糖（CM-壳聚糖）和聚（γ-谷氨酸）（pGlu）与天然存在的化合物京尼平反应，形成具有荧光发射的京尼平交联CM-壳聚糖/pGlu共轭物。京尼平共轭聚合物对葡萄糖的氧化产物葡萄糖酸和过氧化氢（HO）敏感。聚合物的荧光发射被葡萄糖酸和HO猝灭。流体动力学直径的增加以及荧光的猝灭表明，响应葡萄糖酸（而非HO）的刺激，京尼平共轭聚合物自聚集形成纳米颗粒。牛血清白蛋白（BSA）可以负载在自聚集纳米颗粒中，并且在高葡萄糖酸条件下，掺入的BSA会从纳米颗粒中缓慢释放。因此，这种材料被提议作为用于光学传感和蛋白质递送目的的刺激响应材料。
