Sodium caprylate improves intestinal mucosal barrier function and antioxidant capacity by altering gut microbial metabolism.

Short-chain fatty acids (SCFA) produced by gut microbial metabolism have been reported to regulate the immunological response and intestinal health of the host by activating G protein-coupled receptors (GPR). It is unclear whether medium-chain fatty acids (MCFA) improve the intestinal barrier function by activating GPR. This study was conducted to investigate the effects of sodium caprylate and sodium butyrate on antioxidant capacity and intestinal barrier function in IPEC-J2 treated with HO and C57/BL6 mouse models. For and assays, mice and oxidation-damaged IPEC-J2 (NC) were treated with sodium caprylate (SC) and sodium butyrate (PC) to determine intestinal barrier and antioxidant functions of the host. , SC treatment increased the concentrations of glutathione and total antioxidant capacity, and expression of claudin-1 and claudin-3 in oxidation-damaged IPEC-J2 ( < 0.05). , SC treatment improved growth performance, intestinal morphology, expression of claudin-3, and activity of superoxide dismutase in mice ( < 0.05), but decreased interleukin-1β and interferon-γ concentrations in serum compared to the NC group ( < 0.05). Treatment with SC increased the populations of in the ileum and and in the colon, but decreased the abundances of and in the ileum and and in the colon compared with the NC group ( < 0.05). Concentrations of SCFA increased in the SC and PC groups than in the NC group ( < 0.05). Ileal protein expression of GPR 43 in the SC and PC groups was significantly greater than that in the NC group ( < 0.05). In conclusion, our findings confirmed the important role of sodium caprylate in improving intestinal barrier function and gut health by activating GPR 43 through regulating gut microbial metabolism.