Department of Veterinary Public Health and Preventive Medicine, University of Ilorin, Ilorin, Nigeria.
Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Foodborne Pathog Dis. 2022 Jan;19(1):56-61. doi: 10.1089/fpd.2021.0030. Epub 2021 Oct 15.
In recent years, methicillin-resistant (MRSA) has gained interest in veterinary medicine due to its zoonotic potential. Currently, little information is available on the genotypic and virulence characteristics of MRSA isolates detected in Nigerian abattoirs. To better understand the epidemiology of MRSA associated with the abattoir food chain environment in Nigeria, a total of 18 isolates (humans: = 5, slaughter animals: = 5, and environment: = 8), previously typed, were recovered and characterized by cassette chromosome (SCC) typing, and phenotypic and genotypic antimicrobial susceptibility testing. In addition, 10 of the 18 MRSA strains with a new type (t16571) were subjected to multilocus sequence typing. The similarity of strains was analyzed based on the results of the DNA microarray analysis. The 18 MRSA strains harbored two distinct SCC types (IVa and V) and belonged to four clonal clusters (CC1, CC7, CC88, and CC152). All MRSA of the new type t16571 ( = 10) harbored the SCC type IVa. Seven of the MRSA t16571 strains belonged to ST88, while three other strains were assigned to ST3614. The 18 MRSA isolates were categorized into six virulence profiles, and the detection rate for the Panton-Valentine Leukocidin gene was high (33.3%). The antimicrobial susceptibility profiles of the 18 MRSA varied widely between strains, but phenotypic resistance corresponded to relevant resistance genes harbored. The detection of highly similar MRSA strains in slaughter animals, abattoir workers, and the environment underlines the need to use adequate measures at Nigerian abattoirs to prevent further spread and transmission of MRSA to humans or food.
近年来，耐甲氧西林金黄色葡萄球菌（MRSA）因其具有人畜共患潜力而引起了兽医界的关注。目前，有关在尼日利亚屠宰场检测到的 MRSA 分离株的基因型和毒力特征的信息很少。为了更好地了解与尼日利亚屠宰场食物链环境相关的 MRSA 的流行病学，共回收并鉴定了 18 株分离株（人：= 5，屠宰动物：= 5，环境：= 8），这些分离株之前已被分型，通过盒式染色体（SCC）分型、表型和基因型抗菌药物敏感性测试进行了特征描述。此外，对 10 株具有新类型（t16571）的 MRSA 菌株进行了多位点序列分型。基于 DNA 微阵列分析的结果分析了菌株的相似性。根据 DNA 微阵列分析的结果分析了菌株的相似性。18 株 MRSA 菌株携带两种不同的 SCC 类型（IVa 和 V），属于四个克隆群（CC1、CC7、CC88 和 CC152）。所有新类型 t16571（= 10）的 MRSA 均携带 SCC 类型 IVa。7 株 t16571 型 MRSA 属于 ST88，另外 3 株则属于 ST3614。18 株 MRSA 分离株分为 6 种毒力谱，Panton-Valentine Leukocidin 基因的检出率很高（33.3%）。18 株 MRSA 分离株的抗菌药物敏感性谱在菌株间差异很大，但表型耐药性与携带的相关耐药基因相对应。在屠宰动物、屠宰场工人和环境中检测到高度相似的 MRSA 菌株，这强调了需要在尼日利亚屠宰场采取适当措施，以防止 MRSA 进一步传播和传播给人类或食物。
