Department of Veterinary Public Health and Preventive Medicine, University of Abuja, Abuja, Nigeria.
Department of Veterinary Medicine, University of Abuja, Abuja, Nigeria.
Vet Res Commun. 2024 Jun;48(3):1697-1705. doi: 10.1007/s11259-024-10346-8. Epub 2024 Mar 23.
Methicillin-resistant Staphylococcus aureus (MRSA) transmission in livestock, community, and healthcare settings poses a significant public health concern both locally and globally. This study aimed to investigate the occurrence, molecular detection, and antibiogram of the MRSA strain in fresh beef, contact surfaces, and butchers' knives from the four major abattoirs (Karu, Gwagwalada, Deidei, and Kubwa) located in the Federal Capital Territory, Nigeria. A multi-stage sampling technique was used to collect 400 swab samples from butchers' knives (132), fresh beef (136), and contact surfaces (132). Presumptive colonies on mannitol salt agar were subjected to culture, isolation, and biotyping. The antibiogram was carried out via a Kirby-Bauer disk containing eight antibiotics. MRSA was phenotypically confirmed by oxacillin-resistant screening agar base (ORSAB) and genotypically by PCR to detect the presence of the mecA gene. Out of the 400 samples, 47.24% of fresh beef, 37% of contact surfaces, and 64.33% of butchers' knife swabs were Staphylococcus aureus positive. Thirty-two Staphylococcus aureus-positive isolates were confirmed to be MRSA, 50% fresh beef, 28.12% contact surfaces, and 21.87% butcher's knife swabs. MRSA isolates displayed multidrug-resistant traits, with a high resistance of 90.62% against cloxacillin, and a highest susceptibility of 100% to co-trimaxole. The antibiogram showed MRSA strains to be multidrug resistant. Molecular characterisation of the MRSA detected the presence of the mecA gene at a band size of 163 bp in all isolates. Strict hygiene of butchers, and working equipment in meat processing and marketing should be of top priority.
耐甲氧西林金黄色葡萄球菌（MRSA）在牲畜、社区和医疗机构中的传播，无论是在本地还是全球范围内，都构成了重大的公共卫生关切。本研究旨在调查尼日利亚联邦首都辖区（FCT）四个主要屠宰场（Karu、Gwagwalada、Deidei 和 Kubwa）的新鲜牛肉、接触表面和屠夫刀中 MRSA 菌株的发生、分子检测和抗生素敏感性。采用多阶段抽样技术，从屠夫刀（132 把）、新鲜牛肉（136 块）和接触表面（132 个）采集了 400 个拭子样本。甘露醇盐琼脂上的推定菌落经培养、分离和生物分型。通过含有八种抗生素的 Kirby-Bauer 圆盘进行抗生素敏感性试验。MRSA 通过耐苯唑西林筛选琼脂基础（ORSAB）进行表型确认，通过 PCR 检测 mecA 基因的存在进行基因分型确认。在 400 个样本中，47.24%的新鲜牛肉、37%的接触表面和 64.33%的屠夫刀拭子为金黄色葡萄球菌阳性。32 个金黄色葡萄球菌阳性分离株被确认为耐甲氧西林金黄色葡萄球菌，其中 50%来自新鲜牛肉、28.12%来自接触表面、21.87%来自屠夫刀拭子。MRSA 分离株表现出多药耐药特征，对氯唑西林的耐药率高达 90.62%，对复方新诺明的敏感性最高，为 100%。抗生素敏感性试验显示，MRSA 菌株呈多药耐药性。对检测到的 MRSA 的分子特征分析显示，所有分离株的 mecA 基因在 163 bp 大小的条带上均有存在。严格遵守屠夫的个人卫生和在肉类加工和销售过程中使用的工作设备的卫生至关重要。
