Department of Orthopaedic Surgery, BioMedical Engineering, Western Michigan University Homer Stryker M.D. School of Medicine, Kalamazoo, Michigan, USA.
Center for Stem Cell and Regenerative Medicine (CSCRM), The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases (IMM), McGovern Medical School, University of Texas Health Science Center at Houston, Houston, Texas, USA.
J Cell Biochem. 2022 Feb;123(2):147-154. doi: 10.1002/jcb.30164. Epub 2021 Oct 19.
Epigenetics play a fundamental role in induced pluripotent stem cell (iPSC) technology due to their effect on iPSC's reprogramming efficiency and their subsequent role in iPSC differentiation toward a specific lineage. Epigenetics can skew the differentiation course of iPSCs toward a specific lineage based on the epigenetic memory of the source cells, or even lead to acquisition of new cell phenotypes, due to its aberrations during reprogramming. This viewpoint discusses key features of the epigenetic process during iPSC reprogramming/differentiation and outlines important epigenetic factors that need to be considered for successful generation and differentiation of iPSCs for downstream applications.
表观遗传学在诱导多能干细胞（iPSC）技术中起着至关重要的作用，因为它们会影响 iPSC 的重编程效率，以及它们在 iPSC 向特定谱系分化中的后续作用。由于在重编程过程中发生了表观遗传学异常，表观遗传学可以根据源细胞的表观遗传学记忆使 iPSC 的分化过程发生偏差，甚至导致获得新的细胞表型。本文观点讨论了 iPSC 重编程/分化过程中的表观遗传过程的关键特征，并概述了成功生成和分化 iPSC 以用于下游应用所需考虑的重要表观遗传因素。
