Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Department of Urology, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
Bioengineered. 2021 Dec;12(1):8485-8495. doi: 10.1080/21655979.2021.1984006.
The function of tubulin polymerization promoting protein family member 3 (TPPP3) in tumor cells is complicated, and the role of TPPP3 in nasopharyngeal carcinoma (NPC) remains unclear. This study aims to explore the expression of TPPP3 in NPC and its effect on NPC cells. The expression of TPPP3 in NPC tissues and other cancers were analyzed by using the Oncomine and Gene Expression Omnibus (GEO) databases. The mRNA and protein of TPPP3 were detected in NPC tissues by quantitative real-time PCR and immunohistochemistry. Furthermore, TPPP3 was overexpressed in 5-8 F and HONE1 cell lines by lentivirus transfection, and functional analysis of TPPP3 in NPC was evaluated through in vitro experiments. The expression of TPPP3 was significantly down-regulated in NPC tissues and cells. Overexpression of TPPP3 significantly inhibited proliferation of 5-8 F and HONE1 cells in vitro. In addition, overexpression of TPPP3 significantly attenuated the invasion ability of 5-8 F, HONE1 cells in vitro, but have no significant effect on migration ability. Furthermore, TPPP3 overexpression diminished the expression of MMP-2 and MMP-9 mRNA. By analyzing dataset GSE12452, it was interesting that TPPP3 high expression group mainly functioned in B cell receptor signaling pathway, cell cycle and DNA replication. In conclusion, our results suggest that TPPP3 may be considered as an antioncogene, which plays an important role in the occurrence and progression of NPC. TPPP3: tubulin polymerization promoting protein family member 3; NPC: nasopharyngeal carcinoma; GEO: Gene Expression Omnibus; qRT-PCR: quantitative real-time PCR; GFP: green fluorescence protein; MOI, transfected multiplicity of infection; CCK-8: cell counting kit-8; OD: optical density; GSEA: gene set enrichment analysis; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MMP-2: matrix metalloproteinase-2; MMP-9: matrix metalloproteinase-9.
TPPP3 蛋白家族成员 3（TPPP3）在肿瘤细胞中的功能较为复杂，其在鼻咽癌（NPC）中的作用尚不清楚。本研究旨在探讨 TPPP3 在 NPC 中的表达及其对 NPC 细胞的影响。通过 Oncomine 和基因表达综合数据库（GEO）分析 TPPP3 在 NPC 组织和其他癌症中的表达。通过实时定量 PCR 和免疫组织化学检测 NPC 组织中 TPPP3 的 mRNA 和蛋白。此外，通过慢病毒转染在 5-8F 和 HONE1 细胞系中过表达 TPPP3，并通过体外实验评估 TPPP3 在 NPC 中的功能。TPPP3 在 NPC 组织和细胞中的表达显著下调。TPPP3 的过表达显著抑制 5-8F 和 HONE1 细胞的体外增殖。此外，TPPP3 的过表达显著减弱 5-8F、HONE1 细胞的体外侵袭能力，但对迁移能力无明显影响。此外，TPPP3 过表达降低了 MMP-2 和 MMP-9 mRNA 的表达。通过分析数据集 GSE12452，有趣的是 TPPP3 高表达组主要在 B 细胞受体信号通路、细胞周期和 DNA 复制中起作用。综上所述，我们的研究结果表明，TPPP3 可能被视为一种抑癌基因，在 NPC 的发生和发展中发挥重要作用。
