Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
Guizhou Provincial People's Hospital, Guiyang, Guizhou, China.
Sci Rep. 2023 Oct 20;13(1):17960. doi: 10.1038/s41598-023-45233-w.
Tubulin polymerization promoting protein 3 (TPPP3), a member of the tubulin polymerization family, participates in cell progressions in several human cancers, its biological function and the underlying mechanisms in glioblastoma multiforme (GBM) remain unclear. Here, we investigated the role and application value of TPPP3 in gliomas and found that the expression of TPPP3 in glioma was higher than that in normal brain tissue (NBT), and increased with the grade of glioma. Up-regulation of TPPP3 expression in glioblastoma cells confer stronger ability of migration, invasion, proliferation and lower apoptosis in vitro. Inhibition of TPPP3 expression in GBM could reduce the migration, invasion, proliferation and induce the apoptosis of glioblastoma cells. TPPP3 affected the process of EMT by regulating the expression of Snail 1 protein. In clinical data analysis, we found a positive correlation between TPPP3 and Snail1 protein expression levels in glioblastomas. Low TPPP3 expression leads to better survival expectations in glioblastomas patients. The content of this study paves the way for further in-depth exploration of the role of TPPP3 in glioblastoma in the future, and provides new treatment and research directions.
微管蛋白聚合促进蛋白 3（TPPP3）是微管聚合家族的一员，参与多种人类癌症的细胞进展，但其在多形性胶质母细胞瘤（GBM）中的生物学功能和潜在机制尚不清楚。在这里，我们研究了 TPPP3 在神经胶质瘤中的作用和应用价值，发现 TPPP3 在神经胶质瘤中的表达高于正常脑组织（NBT），并且随着神经胶质瘤的分级而增加。在神经母细胞瘤细胞中上调 TPPP3 的表达赋予了更强的迁移、侵袭、增殖能力，体外凋亡率降低。在 GBM 中抑制 TPPP3 的表达可减少神经母细胞瘤细胞的迁移、侵袭、增殖，并诱导其凋亡。TPPP3 通过调节 Snail1 蛋白的表达影响 EMT 过程。在临床数据分析中，我们发现 TPPP3 和 Snail1 蛋白在神经母细胞瘤中的表达水平呈正相关。TPPP3 低表达可使神经母细胞瘤患者的生存预期更好。本研究的内容为今后进一步深入探讨 TPPP3 在神经母细胞瘤中的作用奠定了基础，并为治疗和研究提供了新的方向。
