TPPP3是一种良好的预后指标，可抑制口腔鳞状细胞癌的细胞增殖和迁移。
TPPP3, a Good Prognostic Indicator, Suppresses Cell Proliferation and Migration in OSCC.
作者信息
Xiao Ting, Rahhal Omar, Wang Liping, Deng Zhiyuan, Wang Ran, Xu Xinghuanyu, Qi Lu, Tang Zhangui
机构信息
Hunan Key Laboratory of Oral Health Research, Hunan Clinical Research Center of Oral Major Diseases and Oral Health, Xiangya Stomatological Hospital, Xiangya School of Stomatology, Central South University, Changsha, Hunan, China.
Beijing Nuclear Industry Hospital, Beijing, China.
出版信息
Int Dent J. 2025 Apr;75(2):970-983. doi: 10.1016/j.identj.2024.09.035. Epub 2025 Jan 14.
INTRODUCTION AND AIMS
Oral squamous cell carcinoma (OSCC) is one of the most prevalent malignancy of the head and neck. Early diagnosis of OSCC is difficult and the prognosis has not improved significantly. This study aims to explore the role of tubulin polymerisation promoting protein 3 (TPPP3) in the occurrence and development of OSCC and discover new diagnostic and prognostic markers for OSCC.
METHODS
Using UALCAN, GEPIA, western blot, and quantitative real-time polymerase chain reaction, we studied TPPP3 expression and its relationship with tumour stage. Then, we detected the effect of TPPP3 on OSCC biological functions by CCK-8 and cell scratch assays, as well as correlations between TPPP3 expression and survival of different kinds of head and neck squamous cell carcinoma (HNSC) patients through Kaplan-Meier plotter. Besides, we explored coexpressed genes associated with TPPP3 in HNSC using LinkedOmics and protein-protein interaction networks of TPPP3 using STRING and Cytoscape. Furthermore, we explored possible molecular mechanisms that TPPP3 functions in HNSC using UALCAN, Kaplan-Meier plotter, and TIMER. Finally, we analysed promoter methylation level by UALCAN and mutation by cBioPortal of TPPP3 in HNSC.
RESULTS
TPPP3 was less expressed in OSCC. The TPPP3 expression level was negatively correlated with tumour stage. Furthermore, TPPP3 significantly inhibited OSCC proliferation and migration. Besides, TPPP3 high expression was significantly associated with good prognosis in different kinds of HNSC patients. Additionally, TPPP3 may regulate the occurrence and development of OSCC through the PALMD/PI3K pathway. TPPP3 methylation level in HNSC decreased. Finally, we found that TPPP3 genetic alteration was involved in TPPP3 mRNA expression change in HNSC.
CONCLUSION
TPPP3 functions as a tumour suppressor in OSCC and is associated with good prognosis in HNSC patients. TPPP3 can be used as a potential biomarker for prognosis and diagnosis of OSCC.
CLINICAL RELEVANCE
TPPP3 can be used as a potential biomarker for prognosis and diagnosis of OSCC in clinical practice.
引言与目的
口腔鳞状细胞癌（OSCC）是头颈部最常见的恶性肿瘤之一。OSCC的早期诊断困难，且预后改善不显著。本研究旨在探讨微管蛋白聚合促进蛋白3（TPPP3）在OSCC发生发展中的作用，并发现OSCC新的诊断和预后标志物。
方法
我们使用UALCAN、GEPIA、蛋白质免疫印迹法和定量实时聚合酶链反应，研究了TPPP3的表达及其与肿瘤分期的关系。然后，我们通过CCK-8和细胞划痕试验检测了TPPP3对OSCC生物学功能的影响，并通过Kaplan-Meier生存分析软件分析了TPPP3表达与不同类型头颈部鳞状细胞癌（HNSC）患者生存率之间的相关性。此外，我们使用LinkedOmics探索了HNSC中与TPPP3共表达的基因，并使用STRING和Cytoscape构建了TPPP3的蛋白质-蛋白质相互作用网络。进一步地，我们使用UALCAN、Kaplan-Meier生存分析软件和TIMER探索了TPPP3在HNSC中发挥作用的可能分子机制。最后，我们通过UALCAN分析了TPPP3在HNSC中的启动子甲基化水平，并通过cBioPortal分析了其突变情况。
结果
TPPP3在OSCC中表达较低。TPPP3表达水平与肿瘤分期呈负相关。此外，TPPP3显著抑制OSCC的增殖和迁移。另外，TPPP3高表达与不同类型HNSC患者的良好预后显著相关。此外，TPPP3可能通过PALMD/PI3K途径调节OSCC的发生发展。HNSC中TPPP3的甲基化水平降低。最后，我们发现TPPP3基因改变参与了HNSC中TPPP3 mRNA表达的变化。
结论
TPPP3在OSCC中起肿瘤抑制作用，与HNSC患者的良好预后相关。TPPP3可作为OSCC预后和诊断的潜在生物标志物。
临床意义
在临床实践中，TPPP3可作为OSCC预后和诊断的潜在生物标志物。
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