Departamento de Química, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, Curitiba, PR, Brazil, 81531-980.
Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), Universidade Estadual de Campinas, Av. Alexandre Cazelatto, 999, Vila Betel, Paulínia, SP, Brazil, 13148-218.
Chem Biodivers. 2021 Nov;18(11):e2100495. doi: 10.1002/cbdv.202100495. Epub 2021 Oct 20.
The essential oils (EOs) chemical composition can be affected by several environmental factors, impacting their desired biological activities. In this sense, this work aimed to evaluate the seasonal variation of the chemical composition and antimicrobial activity of Piper caldense and Piper xylosteoides leaves EOs. Their chemical composition was determined by GC/MS and GC-FID analyses, resulting in the identification of eighty compounds. P. caldense EOs were mainly consisted of sesquiterpene hydrocarbons, whereas in P. xylosteoides EOs, monoterpene hydrocarbons were predominant. EOs from both species strongly inhibited B. subtilis (MIC=0.25 mg mL ), while only P. caldense EOs showed strong activity against S. aureus (MIC=0.50 mg mL ). P. caldense spring EO showed the broadest spectrum of antimicrobial action amongst all samples. For each species, PCA seasonally differentiated EOs chemical composition. In addition, as expected, PCA of all samples showed a distinction between the two species. This study has successfully demonstrated the importance of evaluating the seasonal variation of EOs chemical composition and antimicrobial activity in obtaining a product with the desired properties.
植物精油（EOs）的化学成分会受到多种环境因素的影响，从而影响其预期的生物活性。在这个意义上，本工作旨在评估 Piper caldense 和 Piper xylosteoides 叶精油的化学成分和抗菌活性的季节性变化。通过 GC/MS 和 GC-FID 分析对其化学成分进行了测定，结果鉴定了 80 种化合物。P. caldense EOs 主要由倍半萜类化合物组成，而在 P. xylosteoides EOs 中，单萜类化合物占优势。两种植物精油均强烈抑制枯草芽孢杆菌（MIC=0.25 mg/mL），而只有 P. caldense EOs 对金黄色葡萄球菌（MIC=0.50 mg/mL）表现出强烈的活性。P. caldense 春季 EO 在所有样品中表现出最广泛的抗菌作用谱。对于每种植物，PCA 季节性区分了 EOs 的化学成分。此外，如预期的那样，所有样品的 PCA 也区分了这两个物种。本研究成功地证明了评估 EOs 化学成分和抗菌活性季节性变化以获得具有所需特性的产品的重要性。
