Pagotti Mariana C, Candido Ana C B B, Marçal Maria G, Vieira Tatiana M, Groppo Milton, Silva Márcio L A, Ferreira Daniele S, Esperandim Viviane R, Crotti Antônio E M, Magalhães Lizandra G
Núcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, Dr. Armando Salles Oliveira 201, CEP 14404-600, Franca, SP, Brazil.
Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, CEP 14040-901, Ribeirão Preto, SP, Brazil.
Chem Biodivers. 2021 Dec;18(12):e2100678. doi: 10.1002/cbdv.202100678. Epub 2021 Oct 20.
Despite the current treatments against Chagas Disease (CD), this vector-borne parasitic disease remains a serious public health concern. In this study, we have explored the in vitro and/or in vivo trypanocidal and cytotoxic activities of the essential oils (EOs) obtained from Dysphania ambrosioides (L.) Mosyakin & Clemants (Amaranthaceae) (DA-EO), Lippia alba (Mill.) N.E. Brown (Verbenaceae) (LA-EO), and Tetradenia riparia (Hochst.) Codd (Lamiaceae) (TR-EO) grown in Brazil Southeast. DA-EO was the most active against the trypomastigote and amastigote forms in vitro; the IC values were 8.7 and 12.2 μg mL , respectively. The EOs displayed moderate toxicity against LLCMK2 cells, but the DA-EO showed high selectivity index (SI) for trypomastigote (SI=33.2) and amastigote (SI=11.7) forms. Treatment with 20 mg/kg DA-EO, LA-EO, or TR-EO for 20 days by intraperitoneal administration reduced parasitemia by 6.36 %, 4.74 %, and 32.68 % on day 7 and by 12.04 %, 27.96 %, and 65.5 % on day 9. These results indicated that DA-EO, LA-EO, and TR-EO have promising trypanocidal potential in vitro, whereas TR-EO has also potential trypanocidal effects in vivo.
尽管目前有针对恰加斯病（CD）的治疗方法，但这种由媒介传播的寄生虫病仍然是一个严重的公共卫生问题。在本研究中，我们探究了从生长于巴西东南部的土荆芥（藜科）（DA-EO）、白千层（马鞭草科）（LA-EO）和河边香茶菜（唇形科）（TR-EO）中提取的挥发油（EOs）的体外和/或体内杀锥虫及细胞毒性活性。DA-EO在体外对锥鞭毛体和无鞭毛体形式最具活性；IC值分别为8.7和12.2μg/mL。这些挥发油对LLCMK2细胞显示出中度毒性，但DA-EO对锥鞭毛体（SI = 33.2）和无鞭毛体（SI = 11.7）形式表现出高选择性指数（SI）。通过腹腔注射给予20mg/kg DA-EO、LA-EO或TR-EO，持续20天，在第7天分别使寄生虫血症降低6.36%、4.74%和32.68%，在第9天分别降低12.04%、27.96%和65.5%。这些结果表明，DA-EO、LA-EO和TR-EO在体外具有有前景的杀锥虫潜力，而TR-EO在体内也具有杀锥虫作用。
