Oliveira Thaís A S, Vieira Tatiana M, Esperandim Viviane R, Martins Carlos H G, Magalhães Lizandra G, Miranda Mayker L D, Crotti Antônio E M
Department of Chemistry, Faculty of Phylosophy, Sciences, and Letters of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto 14049-900, SP, Brazil.
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Pharmaceuticals (Basel). 2022 Aug 5;15(8):967. doi: 10.3390/ph15080967.
This study aimed to investigate the chemical composition as well as the antibacterial, antiparasitic, and cytotoxic potentialities of the Brazilian root essential oil (CZ-EO) In addition, CZ-EO cytotoxicity to LLCMK adherent epithelial cells was assessed. The major compounds identified in CZ-EO were khusimol (30.0 ± 0.3%), β-eudesmol (10.8 ± 0.3%), α-muurolene (6.0 ± 0.1%), and patchouli alcohol (5.6 ± 0.2%). CZ-EO displayed optimal antibacterial activity against , , , and , with Minimum Inhibitory Concentration (MIC) values between 22 and 62.5 µg/mL and Minimum Bactericidal Concentration (MBC) values between 22 and 400 µg/mL. CZ-EO was highly active against the promastigote and amastigote forms (IC = 7.20 and 16.21 µg/mL, respectively) and the trypomastigote form (IC = 11.2 µg/mL). Moreover, CZ-EO showed moderate cytotoxicity to LLCMK cells, with CC = 565.4 µg/mL. These results revealed an interesting in vitro selectivity of CZ-EO toward the promastigote and amastigote forms (Selectivity Index, SI = 78.5 and 34.8, respectively) and the trypomastigote form (SI = 50.5) compared to LLCMK cells. These results showed the promising potential of CZ-EO for developing new antimicrobial, antileishmanial, and antitrypanosomal drugs.
本研究旨在调查巴西根部精油（CZ-EO）的化学成分以及抗菌、抗寄生虫和细胞毒性潜力。此外，还评估了CZ-EO对LLCMK贴壁上皮细胞的细胞毒性。在CZ-EO中鉴定出的主要化合物为库西莫尔（30.0±0.3%）、β-桉叶醇（10.8±0.3%）、α-衣兰油烯（6.0±0.1%）和广藿香醇（5.6±0.2%）。CZ-EO对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌和白色念珠菌表现出最佳抗菌活性，最低抑菌浓度（MIC）值在22至62.5μg/mL之间，最低杀菌浓度（MBC）值在22至400μg/mL之间。CZ-EO对前鞭毛体和无鞭毛体形式（IC50分别为7.20和16.21μg/mL）以及锥鞭毛体形式（IC50为11.2μg/mL）具有高度活性。此外，CZ-EO对LLCMK细胞表现出中度细胞毒性，CC50为565.4μg/mL。这些结果揭示了CZ-EO对前鞭毛体和无鞭毛体形式（选择性指数，SI分别为78.5和34.8）以及锥鞭毛体形式（SI为50.5）相对于LLCMK细胞具有有趣的体外选择性。这些结果表明CZ-EO在开发新型抗菌、抗利什曼原虫和抗锥虫药物方面具有广阔的潜力。
