Gao Kai, Wang Xiaoyan, Wang Zhonghua, He Lijiao, Lin Jiayu, Bai Zhenzu, Jiang Kai, Huang Shan, Zheng Weijia, Liu Long
Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, Hunan 410073, People's Republic of China.
Kunming Sanatorium, Kunming, Yunnan 650000, People's Republic of China.
Biomed Mater. 2021 Nov 2;16(6). doi: 10.1088/1748-605X/ac3146.
Collagen and chitosan are two different kinds of natural biodegradable polymers commonly used in the regeneration of bone defects. Mesoporous bioactive glass (MBG) is a type of favorable bone filler which can effectively constitute an enlarged microenvironment to facilitate an exchange of important factors between the cells and scaffolds. Here we prepared a collagen-chitosan-MBG (C-C-MBG) scaffold which displayed significantly increased proliferation, differentiation and mineralization in bone mesenchymal stem cells (BMSCs). Additionally, we found that the scaffold can stimulate extra-cellular signal regulated kinase 1/2 (Erk1/2) activated Runx2 pathway, which is the predominant signaling pathway involved in osteoblast differentiation. Consistently, we observed that the scaffold can markedly enhance the expression of(), and, which are important osteoblastic marker genes implicated in the process of osteoblast differentiation. Therefore, we conclude that the composite scaffold can significantly promote the differentiation of BMSCs into osteoblasts by activating Erk1/2-Runx2 pathway. Our finding thereby implies that the C-C-MBG scaffold can possibly act as a potential biomaterial in the bone regeneration.
胶原蛋白和壳聚糖是常用于骨缺损再生的两种不同类型的天然可生物降解聚合物。介孔生物活性玻璃（MBG）是一种良好的骨填充材料，它可以有效地构建一个扩大的微环境，以促进细胞与支架之间重要因子的交换。在此，我们制备了一种胶原蛋白-壳聚糖-MBG（C-C-MBG）支架，该支架在骨间充质干细胞（BMSCs）中显示出显著增加的增殖、分化和矿化。此外，我们发现该支架可以刺激细胞外信号调节激酶1/2（Erk1/2）激活的Runx2途径，这是参与成骨细胞分化的主要信号通路。一致地，我们观察到该支架可以显著增强（）、和的表达，这些是参与成骨细胞分化过程的重要成骨细胞标记基因。因此，我们得出结论，复合支架可以通过激活Erk1/2-Runx2途径显著促进BMSCs向成骨细胞的分化。我们的发现由此表明，C-C-MBG支架可能作为骨再生中的一种潜在生物材料。
