Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, 410073, Hunan, People's Republic of China.
Shaoyang No. 11 Middle School, Shaoyang, 422000, Hunan, People's Republic of China.
Biotechnol Lett. 2020 Feb;42(2):321-328. doi: 10.1007/s10529-019-02773-4. Epub 2019 Nov 28.
Mesoporous bioactive glass (MBG) has good biocompatibility without immune reaction after implanting into tissue as biomaterial which was used in the treatment of bone defect. Genistein (G), a phytoestrogen, could be used in the treatment of osteoporosis in postmenopausal women.
Here, we report that MBG with large pores (MBG-L) and MBG-L adsorbed with G (MBG-L/G) sustained-release G could enhance osteoblast differentiation and matrix mineralization. Interestingly, we observed that MBG-L enhanced the formation of bone-like deposit and Ca deposition in vitro. In the other side, we also found that MBG-L/G substrate could promote osteoblast differentiation and matrix mineralization through Erk activated Runx2 pathway. Interestingly, the expression of osteoblast-specific marker gene Osteopontin (Opn) was also increased in MC3T3-E1 cells cultured on MBG-L/G substrate.
We conclude that MBG-L/G is a potential biomaterial for the treatment of bone defect.
介孔生物活性玻璃（MBG）作为生物材料植入组织后具有良好的生物相容性，不会引起免疫反应，可用于治疗骨缺损。染料木黄酮（G）是一种植物雌激素，可用于治疗绝经后妇女的骨质疏松症。
在这里，我们报告说，具有大孔的 MBG（MBG-L）和吸附 G 的 MBG-L（MBG-L/G）缓释 G 可以增强成骨细胞分化和基质矿化。有趣的是，我们观察到 MBG-L 增强了体外骨样沉积物和 Ca 沉积的形成。另一方面，我们还发现 MBG-L/G 基底通过激活 Erk 的 Runx2 通路促进成骨细胞分化和基质矿化。有趣的是，在 MBG-L/G 基底上培养的 MC3T3-E1 细胞中，成骨细胞特异性标记基因骨桥蛋白（Opn）的表达也增加了。
我们得出结论，MBG-L/G 是一种治疗骨缺损的潜在生物材料。
