Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, Hunan 410073, PR China.
Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, Hunan 410073, PR China.
Int J Biol Macromol. 2022 May 31;208:136-148. doi: 10.1016/j.ijbiomac.2022.03.056. Epub 2022 Mar 14.
Mesoporous bioactive glass (MBG) is a potential biomedical material in bone defect repairment because of its bioactivity, biocompatibility, and osteoinduction properties. Here we report that Mg-doped MBG scaffold with 3:1 Ca/Mg ratio (MBG-Ca/Mg-3) is good for MC3T3-E1 osteoblast differentiation and mineralization. Mimicking bone extracellular matrix structure by electrospinning, we used MBG-Ca/Mg-3 adsorbed with Osteocalcin-Osteopontin-Biglycan (OOB), a new unique matrix fusion protein, to form OOB@MBG-Ca/Mg-3 scaffold, which has multifunctional ability in calvarial bone defect repairment in vivo. Intriguingly, we found that OOB@MBG-Ca/Mg-3 scaffold increases the expression of osteoblastic marker genes, including bone morphogenetic protein (Bmp2), osteopontin (Opn), Osterix, Runx2 through activation of ERK1/2. We concluded that OOB@MBG-Ca/Mg-3 scaffold promotes osteoblast differentiation and mineralization through ERK1/2 pathway and it can also enhance bone formation in vivo, which provides a new biomaterial in bone tissue engineering.
介孔生物活性玻璃（MBG）是一种有潜力的生物医学材料，可用于骨缺损修复，因为它具有生物活性、生物相容性和骨诱导特性。在这里，我们报告说，具有 3:1 Ca/Mg 比例的 Mg 掺杂 MBG 支架（MBG-Ca/Mg-3）有利于 MC3T3-E1 成骨细胞的分化和矿化。我们通过静电纺丝模拟骨细胞外基质结构，使用吸附有骨钙素-骨桥蛋白-核心蛋白聚糖（OOB）的 MBG-Ca/Mg-3 支架形成 OOB@MBG-Ca/Mg-3 支架，该支架在体内的颅骨骨缺损修复中具有多功能能力。有趣的是，我们发现 OOB@MBG-Ca/Mg-3 支架通过激活 ERK1/2 增加了成骨细胞标记基因的表达，包括骨形态发生蛋白 2（Bmp2）、骨桥蛋白（Opn）、osterix 和 Runx2。我们得出结论，OOB@MBG-Ca/Mg-3 支架通过 ERK1/2 通路促进成骨细胞的分化和矿化，并且还可以增强体内的骨形成，这为骨组织工程提供了一种新的生物材料。
