Suzuki Y, Tsuda M, Takiya S, Hirose S, Suzuki E, Kameda M, Ninaki O
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9522-6. doi: 10.1073/pnas.83.24.9522.
Six cell-free extracts have been used to characterize the nature of DNA signals and trans-acting factors responsible for the transcription enhancement of the Bombyx mori fibroin gene. The upstream element of the fibroin gene involved in the enhancement can be divided into two regions. The proximal region, -72 to -32, is recognized as a common enhancing signal by all B. mori extracts from the posterior silk gland, the middle silk gland, the ovarian tissue, and an embryonic cell line. It is weakly recognized by an Antheraea silkworm cell line extract but not by a HeLa cell extract. The distal region, -238 to -73, appears to be a tissue-specific enhancing signal that is recognized more effectively by the posterior silk gland extract than by the middle silk gland extract. These observations suggest that the use of these cell-free systems can offer a means for the biochemical characterization of the trans-acting factors involved in the tissue-specific regulation of the fibroin gene.
六种无细胞提取物已被用于表征负责家蚕丝素基因转录增强的DNA信号和反式作用因子的性质。参与增强作用的丝素基因上游元件可分为两个区域。近端区域，-72至-32，被来自后部丝腺、中部丝腺、卵巢组织和胚胎细胞系的所有家蚕提取物识别为共同的增强信号。它被柞蚕细胞系提取物微弱识别，但不被HeLa细胞提取物识别。远端区域，-238至-73，似乎是一种组织特异性增强信号，后部丝腺提取物比中部丝腺提取物更有效地识别它。这些观察结果表明，使用这些无细胞系统可以为参与丝素基因组织特异性调控的反式作用因子的生化表征提供一种方法。
