Mine E, Sakurai H, Izumi S, Tomino S
Department of Biology, Tokyo Metropolitan University, Japan.
Mol Biotechnol. 1997 Aug;8(1):79-88. doi: 10.1007/BF02762341.
This article describes the development of cell-free transcription systems from the cultured cells and fat body tissues of a Lepidpteran insect, the silkworm, Bombyx mori. Detailed protocols are provided for the culture of a B. mori cell line, rearing larvae, preparation of whole cell as well as nuclear extract and conditions for in vitro transcription of cloned plasma protein gene templates.
本文描述了从一种鳞翅目昆虫家蚕的培养细胞和脂肪体组织中开发无细胞转录系统的过程。提供了详细的方案，用于家蚕细胞系的培养、幼虫饲养、全细胞以及核提取物的制备，以及克隆血浆蛋白基因模板的体外转录条件。
